RESUMO
Objective To determine the content icariin in the extract of Gushuling. Methods HPLC method was performed on Luna C18 column (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of acetonitrile- water (30∶70) and the UV detective wavelength was set at 270 nm. Results The curve of icariin was linear within 0.416～2.496 ?g, Y=2.0?106X-53 810 (r =0.999 9). Conclusion HPLC method is simple, rapid and accurate, and it is suitable for ananlysis of icariin content in extract of Gushuling .
RESUMO
OBJECTIVE:To determine the related substance in cloperastine hydrochloride by HPLC.METHODS:The analysis was conducted on Agilent C18 column with the mobile phase consisted of 0.02 mol?L-1 KH2PO4-methanol(30∶70,triethlaminel 1.0 mL,adjusted to pH=3.5 with phosphoric acid) at a flow rate of 1.0 mL?min-1.The detection wavelength was 225 nm;the column temperature was set at room temperature,and the injection volume was 20 ?L.RESULTS:Effective segregation was achieved for the related substance from the chief chromatographic peak.The total peak area of the related substance in the sample was 0.13%～0.26% that of the main constituent of its reference substance(own control solution).CONCLUSION:The method is simple,accurate and specific,and applicable for the quality control of the related substance in cloperastine hydrochloride.