The effect of CD151 on Akt-Ser~(473) phosphorylation and human umbilical veine endothelial cell proliferation / 医学研究生学报

Objective:To investigate the the effect of CD151 on Akt-Ser473 and human umbilical veine endothelial cell proliferation.Methods:To construct pAAV-CD151 and transiently transfect to HUVEC(human umbilical veine endothelical) mediated with Lipofectamine 2000.HUVEC were assigned to control group,GFP group,CD151 group.After transfection,the expression of CD151,Akt and phospho-Akt were measured by western blot.Proliferation of HUVEC were measured by MTT colorimetric assay.Results:①The expression of CD151,phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group(P

The effects of CD151 on the expression of phosphoinositide 3-kinase and the proliferation of human umbilical vein endothelial cells in vitro / 中国医师杂志

Objective To investigate the effects of CD151 on the expression of phosphoinositide 3-kinase(PI3K) and the proliferation of human umbilical vein endothelial cells(HUVEC) in vitro.Methods The plasmid p-AAV-CD151 was constructed and transfected into HUVEC.The proliferation activity of HUVEC was measured by MTT colorimetric assay.The expressions of CD151 and PI3K were tested by Western Blot.Results After transfection with p-AAV-CD151,the expressions of CD151 and PI3K were significantly increased compared with those of non-transfected group and p-AAV-GFP transfected group(P