Gasless submental approach endoscopic removal of thyroglossal cyst / 中华耳鼻咽喉头颈外科杂志

Objective: To explore the safety and feasibility of gasless submental approach endoscopic removal of thyroglossal cyst. Methods: This work prospectively included the clinical data of 13 patients who underwent the gasless submental approach endoscopic removal of thyroglossal cyst at the Department of Otolaryngology, the First Affiliated Hospital of Chongqing Medical University from August 2021 to February 2022. The operative time, bleeding volumes, postoperative complications, subjective pain levels, satisfaction with incisional scars, neck and facial deformities, and recurrences were prospectively evaluated by visual analogue scale(VAS) score. Results: All of 13 patients were successfully treated using this new technique. The patients had a median age of 38 years, ranging from 24 to 59 years, comprising of 3 males and 10 females. The length of the submental incision was about 3 cm and the median of operation time was 135 minutes. Postoperative complications were minimal. The median volume of blood loss was 10 ml. Surgical site swelling existed in 1 case and dysphagia for more than 1 week occurred in 2 cases. Patients were discharged from the hospital with a median of 3 days after surgery. On a VAS of 0-10 scores, the pain had a median of 2 on the first day after surgery, and the satisfaction with incision scars and neck and facial deformities showed a median of 8 at 6 months after surgery. There were no recurrences during the follow-up period of 9-15 months. Conclusion: Gasless submental approach endoscopic removal of thyroglossal cyst may be a reliable new surgical method that is safe and has cosmetic advantages.

Effect of NFBD1 gene silencing by RNA interference on radiosensitivity of nasopharyngeal carcinoma CNE-1 cells / 肿瘤

Objective: To investigate the effect of nuclear factor with BRCT domains protein 1 (NFBD1) gene silencing by short hairpin RNA (shRNA) interference on the radiosensitivity of human nasopharyngeal carcinoma CNE-1 cells. Methods: The CNE-1 cells were transfected with NFBD1-shRNA by method of lentiviral infection, the stably transfected cells were selected by puromycin, and the mRNA and protein levels of NFBD1 in CNE-1 cells were detected by real-time fluorogenic quantitative-PCR (RFQ-PCR) and Western blotting, respectively. For the examination of the effect of NFBD1 depletion on the radiosensitivity of CNE-1 cells, the flow cytometry was used to calculate the apoptotic rate and cell cycle distribution, and the survival fraction of the cells was measured via colony formation assay. Results: After transfection with NFBD1-shRNA, the mRNA and protein levels of NFBD1 in CNE-1 cells were decreased obviously. The percentage of the cells in G2/M phase and the apoptotic rate of CNE-1 cells in NFBD1-shRNA group were increased after ionizing radiation (6 MeV) as compared with those of the negative control (NC)-shRNA group (P < 0.05). The survival fraction of CNE-1 cells in NFBD1-shRNA group was significantly reduced after radiation as compared with that of the NC-shRNA group (P < 0.05). Conclusion: Lentivirus-mediated shRNA targeting NFBD1 can steadily silence the expression of NFBD1 gene and enhance the radiosensitivity of CNE-1 cells. Copyright © 2014 by TUMOR.