Effects of mycorrhizal planting on small molecular chemical components of Dendrobium officinale / 中国中药杂志

This study aimed to provide a scientific basis for the application of the mycorrhizal planting technology of Dendrobium officinale by investigating the effects of mycorrhizal planting on the fingerprints of D. officinale and the content of six chemical components. Seventeen samples of D. officinale under mycorrhizal and conventional planting were collected from four regions, such as Jinhua of Zhejiang. The HPLC fingerprints were established to evaluate the similarity of the samples. The content of six chemical components of the samples was determined by HPLC. There were 15 common peaks in the fingerprints, and five of them were identified by marker compounds, which were naringenin, 4,4'-dihydroxy-3,5-dimethoxybibenzyl, 3,4'-dihydroxy-5-methoxybibenzyl, 3',4-dihydroxy-3,5'-dimethoxybibenzyl(gigantol), and 3,4-dihydroxy-4',5-dimethoxybibenzyl(DDB-2). The similarities of the fingerprints of mycorrhizal and conventional planting samples and the control fingerprint were in the ranges of 0.733-0.936 and 0.834-0.942, respectively. The influences of mycorrhizal planting on fingerprints were related to planting regions, the germplasm of D. officianle, and the amount of fungal agent. The content of six chemical components in the samples varied greatly, and the content of DDB-2 was the highest, ranging from 69.83 to 488.47 μg·g~(-1). The mycorrhizal planting samples from Chongming of Shanghai and Taizhou of Jiangsu showed an increase in the content of 5-6 components, while samples from Zhangzhou of Fujian and Jinhua of Zhejiang showed an increase in the content of 1-2 components. The results showed that mycorrhizal planting technology did not change the chemical profile of small molecular chemical components of D. officinale, but affected the content of chemical components such as bibenzyls, which has a good application prospect.

Chemical constituents of Dendrobium devonianum / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Dendrobium devonianum. METHODS: The compounds were isolated and purified by means of column chromatography with silica gel, Sephadex LH-20 and prep- HPLC. Their structures were identified on the basis of physicochemical parameters and spectroscopic data comparison with standard literature data. RESULTS: Twenty-five compounds were isolated from the 95% ethanol extract of D. devonianum, and identified as 3-hydroxy-4', 5-dimethoxybibenzyl(1), tristin(2), 3, 4', 5-trihydroxybibenzyl(3), N-trans-coumaroyltyramine(4), N-trans- caffeoyltyramine(5), p-hydroxyphenylpropionyltyramine(6), N-cis-coumaroyltyramine(7), 2, 5-dihydroxy-4-methoxy-9, 10-dihydrophenanthrene(8), 2, 4, 7-trihydroxy-9, 10-dihydrophenanthrene(9), 4', 5, 7-trihydroxy-flavonol(10), dihydroconifery acetate(11), ethyl 4-hydroxycinnamate(12), p-hydroxycinnamic acid(13), p-hydroxyphenylpropionic acid(14), 4-hydroxybenzoic acid(15), protocatechuic acid(16), erigeside II(17), citrusin C(18), dihydrosyringin(19), leonuriside A(20), adenosine(21), β-sitosterol(22), daucosterol(23), (+)-syringgaresinol-O-β-D-glucopyranoside(24) and sucrose(25). CONCLUSION: Compounds 1-21 and 24-25 are obtained from this plant for the first time. Compound 1 is isolated from the Dendrobium genus for the first time. Compounds 5, 7, 11, 12 and 18 are obtained from Orchidaceae for the first time.

Research and application of Dendrobium officinale protocorm/protocorm-like bodies / 中国药学杂志

OBJECTIVE: To review the research progress of Dendrobium officinale protocorni/protocorm-like bodies according to relevant references at home and abroad in recent len years, and to explore its development prospect. METHODS: Based on the searched literatures, the research progress in the aspects of culture techniques, chemical constituents and pharmacological effects were summarized. RESULTS AND CONCLUSION: There had been a lot of researches on D. officinale protocorm/PLBs culture techniques, and D. officinale protocorm/PLBs could be used for resource regeneration and production of active polysaccharides, but its material basis and pharmacological activity still need to be further studied.

Study on chemical components of Dendrobium officinale protocorm / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Dendrobium officinale protocorm. METHODS: The compounds were isolated and purified by means of column chromatography, and their structures were identified on the basis of spectroscopic data and comparison with standard literature data. RESULTS: Eight compounds were isolated from the(CH3)2CO, CHCl3 and 95% EtOH fractions of the EtOH extract, and identified as 1-O-p-feruloyl-β-D-glucopyranoside(I), arillatose B(II),4-β-D-glucopyranosy-loxy)benzyl alcohol(III), 4-hydroxymethyl-2,6-dimethoxyphenyl-β-D-glucopyranoside(IV), n-hexatriacontanoic acid(V), n-heptaco-sanol(VI), β-sitosterol(VII), and cycloart-23-ene-3β,25-diol(VIII). CONCLUSION: All these compounds were isolated from the protocorms of D. officinale for the first time. Compounds I, II, III, IV, and VIII were obtained from Dendrobium genus for the first time.