RESUMO
<p><b>OBJECTIVE</b>To detect the expression of Per2 in non-small cell lung cancer (NSCLC), and analyze its clinical significance.</p><p><b>METHODS</b>The expression of Per2 was determined in 60 NSCLC and 20 normal lung tissues by immunohistochemical assay, and the relationship between Per2 expression and clinicopathological features was analyzed.</p><p><b>RESULTS</b>The positive expression rates of Per2 in NSCLC and normal lung tissues were 71.7% and 95.0%, respectively (P < 0.05). The expression of Per2 in NSCLC was correlated with pathological differentiation and TNM stage (P < 0.05).</p><p><b>CONCLUSION</b>The expression of Per2 in NSCLC is decreased. The negative expression of Per2 may contribute to the development and invasion in NSCLC.</p>
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Pulmonar de Células não Pequenas , Metabolismo , Patologia , Cirurgia Geral , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares , Metabolismo , Patologia , Cirurgia Geral , Metástase Linfática , Gradação de Tumores , Estadiamento de Neoplasias , Proteínas Circadianas Period , Metabolismo , FumarRESUMO
<p><b>OBJECTIVE</b>To study the formulation and preparation of ampelopsin liposomes and evaluate their quality.</p><p><b>METHOD</b>The liposomes were prepared by a film-ultrasonic dispersion technique. Served as quota with the entrapment ratio and appearance and diameter of the liposomes, the optimal formulation and preparation were selected by means of an uniform design test. The appearance of liposomes was observed by micrography. The diameter and electric charge of surface were determined by granularity mensuration instrument. The entrapment ratio and the leakage rate of ampelopsin liposome were determined by means of dialyze. The content of ampelopsin was determined by UV.</p><p><b>RESULT</b>The result of electron micrography and the size distribution showed that the liposomes were similar to spherical small unilamellar vesicles. The mean diameter was (258.2 +/- 51.2) nm and the electric charge of surface is 19.0 mV. The entrapment ratio of ampelopsin liposomes was 62. 3% and the lecithoid oxidative rate was 0.83% (n = 3).</p><p><b>CONCLUSION</b>The selected formulation and preparation of ampelopsin liposomes is efficient and practicable.</p>