Mechanical phenotype during early differentiation of human mesenchymal stem cells by quantitative measurement of peak force in atomic force microscopy / 第二军医大学学报

Objective To establish a technique for sensitively reflecting the micro-mechanical changes of human mesenchymal stem cells (hMSCs) in their early differentiation process by mechanical phenotype. Methods Based on atomic force microscopy (AFM), peak force quantitative nanomechanical mapping (PF-QNM) technique was used to measure the nanomechanical spectroscopy of hMSCs during differentiation induced by different concentrations of lithium chloride. Results The nanomechanical spectroscopy of hMSCs induced by 4 mmol/L and 30 mmol/L lithium chloride was significantly different after 48 h, while there was significant difference in Young’s modulus after 72 h. Conclusion Nanomechanical spectroscopy is superior to Young' modulus in reflecting the change of mechanical properties of hMSCs in their early differentiation phase. Mechanical phenotype based on nanomechanical spectroscopy can be used as a physical biomarker to identify the hMSCs at their early differentiation stage.

A family with familial dysalbuminaemic hyperthyroxinaemia / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To report a family of familial dysalbuminaemic hyperthyroxinaemia(FDH).</p><p><b>METHODS</b>Four members, including the female proband, mother, daughter and brother, went through the measurement of thyroid hormone and thyroid-stimulating hormone (TSH). Electrophoretic analysis of the patient's serum proteins was carried out after the patient's serum being incubated with fluorescein isothiocyanate (FITC) labeled thyroxine(T4), The point mutation of Alb gene was determined in all members.</p><p><b>RESULTS</b>The measurements of thyroid hormane and TSH showed that in three members (the proband, her mother and her daughter), the total thyroxine(TT4) serum level was high, the total triiodothyronine(TT3), FT4, FT3 and TSH serum levels were normal. And the enhanced albumin binding of fluorescenced T4 by electrophoresis showed a mutation transition 653 G-->A on DNA coding region of albumin. But in the proband's brother, the thyroid function and the results of electrophoresis of thyroxine-binding protein and determination of albumin gene were normal.</p><p><b>CONCLUSION</b>A family with FDH in China is firstly reported here, a mutation at albumin gene DNA coding region 653G-->A causing enhanced albumin binding of T4 results in high T4 level.</p>