Clinical Observation of Baizhu Shengjiang Decoction Combined with Prucalopride Succinate Tablets for Treatment of Chronic Functional Constipation / 广州中医药大学学报

Objective To observe the therapeutic effect of Baizhu Shengjiang Decoction combined with Prucalopride Succinate Tablets for chronic functional constipation. Methods Sixty patients with chronic functional constipation were randomly divided into treatment group and control group, 30 cases in each group. The treatment group was treated with Baizhu Shengjiang Decoction combined with Prucalopride Succinate Tablets orally, and the control group was treated with oral administration of Prucalopride Succinate Tablets alone. The therapeutic effects on traditional Chinese medicine (TCM) symptoms and constipation, and the follow-up therapeutic effect of the two groups were evaluated after treatment. During the three months after the completion of treatment course, the recurrence of constipation was monitored. Results (1) The cure rate of the treatment group was 90.0％ and that of the control group was 60.0％, indicating that the therapeutic effect on TCM symptoms in the treatment group was stronger than that of the control group(P < 0.05).(2) In respect of the relief of constipation, the markedly effective rate was 93.3％ in the treatment group and was 50.0％ in the control group, and the overall effective rate was 100.0％ in the treatment group and was 73.3％ in the control group, the difference being significant between the two groups(P<0.05 or P<0.01).(3) During the treatment, the percentage of patients with weekly completely spontaneous defecation frequency ≥3 was 86.7％ in the treatment group, and was 53.3％ in the control group, the difference being significant (P < 0.05). (4) The results of 3-month follow-up after the completion of treatment course showed that one case from the treatment group had the recurrence of constipation, and the recurred cases from the control group were 6, the difference being significant (P < 0.05). Conclusion Oral use of Baizhu Shengjiang Decoction combined with Prucalopride Succinate Tablets exerts certain therapeutic effect for chronic functional constipation, which is effective both on relieving constipation symptoms and on reducing recurrence rate.

Effect of contracted learning on rehabilitation training to patients with stroke / 现代临床护理

Objective To explore the effect of contract learning on rehabilitation training to patients with stroke . Methods According to the random number table, 120 stroke patients were randomly divided in equal number into control group and observation group. The patients in the control group were treated with conventional nursing during hospitalization and health guidance during follow-up. The patients in the observation group were given rehabilitation training according to contracted learning during the period of hospitalization and follow-up. The time was 6 months. The quality of life was compared between the two groups of patients before and after the intervention, 1 months after discharge, 6 months after discharge. Results After six months of intervention, the 7 dimensions of quality of life, including daily life ability, hand function, mobility, strength, emotion, communication and participation in the intervention group were significantly better than those of the control group (P<0.001). The 6 dimensions of quality of life, including daily life ability, hand function, mobility, strength, emotion, and communication of the control group were significantly better than before the intervention (P<0.001). Conclusion For stroke patients, the rehabilitation training under the learning contract can improve the effect of rehabilitation training so as to improve the quality of life and the prognosis.

Dynamic variation patterns of aconitum alkaloids in daughter root of Aconitum carmichaelii [Fuzi] in the decoction process based on the content changes of nine aconitum alkaloids by HPLC- MS- MS

The chemical components in the decoctions of Chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. In this work, a high-performance liquid chromatography [HPLC] coupled with electrospray ionization [ESI] tandem mass spectrometry method was developed to explore dynamic variation patterns of aconitum alkaloids in Fuzi during the process of decocting aconite root. The fragmentation patterns of aconitum alkaloids using ESI and collision-induced dissociation [CID] techniques were reported. This assay method was validated with respect to linearity [r[2] > 0.9950], precision, repeatability, and accuracy [recovery rate between 94.6 and 107.9%].The result showed that the amounts of aconitum alkaloids in the decoction at different boiling time varied significantly. In the decoction process, the diester- type alkaloids in crude aconite roots have transformed into Benzoylaconines or aconines

Role of coaptation imaging of mitral valve on ischemic mitral regurgitation by real-time three-dimensional transesophageal echocardiography / 中华超声影像学杂志

Objective To explore the impact of morphological changes of mitral valve coaptation on ischemic mitral regurgitation (IMR),and observe coaptation image of mitral valve in systole in patients with IMR by using real-time three-dimensional transesophageal echocardiography (RT3D-TEE).Methods RT3D-TEE was performed on 112 patients with IMR and 38 patients without MR as the control.Patients with IMR were divided into group of mild IMR (n =46),group of moderate IMR (n =45) and group of severe IMR (n =21),according to the severity of mitral regurgitation.Mitral valve quantification (MVQ)software of Qlab 8.1 was used for image post-processing of RT3D-TEE.The coaptation parameters was measured,included length of anterior combination(LCA3dlf),length of posterior combination(LCP3dlf),area of anterior combination(A3DTANT) and area of posterior combination (A3DTPOST) at end-systole.Then,these coaptation parameters of mitral valve were compared statistically.Logistic regression assessing was applied for analyzing the results.Results Coaptation line of anterior and posterior leaflets was almost same in normal subjects.With increasing of mitral regurgitation,a tendency to prolong in LCA3dlf and LCP3dlf were observed in IMR (P ＜0.05).Compared to that in mild and moderate IMR groups,besides A3DTANT and A3DTPOST,LCA3dlf and LCP3dlf were also increased significantly in severe IMR group (P ＜0.05).LCP3dlf was an independent correlation factor of IMR by Logistic regression analysis.The sensitivity was 73.3％ and the specificity was 94.7％ when LCP3dlf≥32 mm was regarded as cut-off point,and the area under the ROC curve was 0.949 (0.900～0.999).Conclusions Abnormal coaptation of anterior and posterior leaflets of mitral valve might be one of main cause of IMR.Involution structure of mitral valve before surgery is helpful for surgeon to select an optical surgical procedure and evaluate effects of treatment in patients with IMR.

Spatial distribution of wall shear stress in the common carotid artery of type 2 diabetes mellitus patients / 中华超声影像学杂志

Objective To investigate the wall shear stress(WSS) in the common carotid artery of type 2 diabetes mellitus(DM) patients,and analyzed the spatial distribution of WSS by using quantitative visualization of blood flow shear stress analysis software.Methods Eighteen male type 2 DM subjects were enrolled as DM group and 18 age-matched healthy subjects were selected as control group.None of the participants was hypertensive,hyperlipidemic or a cigarette smoker.Intimal-medial thickness (IMT),number and size of plaques in the common carotid artery were evaluated by high-resolution echo-Doppler.Color Doppler flow images of common carotid arteries in the two groups were extracted from DICOM files.WSS in the common carotid arteries was calculated by shear stress visualization quantitative analysis software,and the corresponding spatial distribution maps of WSS were designed.Results WSS of the common carotid arteries in the control group were ranged from 4 to 14 dyne/cm2.WSS of the common carotid arteries in the DM group were ranged from 2 to 8 dyne/cm2.Compared to mean WSS value [(6.96 ± 1.17)dyne/cm2] of common carotid arteries in the control group,mean WSS value [(3.14 ± 0.79)dyne/cm2] of common carotid arteries in the DM group was significantly lower (t =9.380,P =0.000).Six diabetic participants had a plaque in one carotid artery and no lesions in the contralateral carotid.Among these subjects,mean WSS was significantly lower in the side with lesion (t =7.324,P =0.000).Therefore,IMT of common carotid arteries in participants was significantly inversely related to WSS (r =-0.76,P ＜0.01).Conclusions The common carotid arteries of DM patients are more prone to atherosclerosis which is associated with reduction of WSS.The hemodynamic profile might represent an additional factor contributing to the increased prevalence and severity of carotid atherosclerosis in diabetic patients compared with general population.

Study on distributions of low wall shear stress in common carotid arteries of patients with intima-media thickening / 中华超声影像学杂志

Objective To measure wall shear stress (WSS) in common carotid arteries of normal subjects and patients with intima-media thickening and analyze the spatial distribution of low wall shear stress by quantitative analysis of blood flow shear stress visualization software.Methods Color Doppler flow images of common carotid arteries in normal subjects (control group) and patients with intima-media thickening (study group) were extracted from DICOM files.Two-dimensional distribution images of WSS,three-dimensional distribution images of WSS and the value of WSS in the common carotid arteries were obtained by this new algorithm.Then,the data and images of WSS were analyzed.Results WSS in the common carotid arteries of the control group were ranged from 4 to14 dyn/cm2.WSS in the common carotid arteries of the study group were ranged from 2 to 6 dyn/cm2.Compared to mean WSS values [(6.91 ±1.20) dyn/cm2] in common carotid arteries of the control group,mean WSS values [(2.87 ± 0.59)dyn/cm2] in common carotid arteries of the study group was significantly lower (t =17.828,P =0.000).Conclusions Low WSS areas have been emerged in local common carotid arteries in the patients with intima-media thickening.The reduction of WSS caused by local hemodynamic change might be related to common carotid artery intima media thickening.

Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.</p><p><b>METHOD</b>By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.</p><p><b>RESULT</b>Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).</p><p><b>CONCLUSION</b>This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.</p>

Optimization of a HPLC determination method for Evodia rutaecarpa / 中国中药杂志

A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.

Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice / 中国中药杂志

<p><b>OBJECTIVE</b>To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.</p><p><b>METHOD</b>HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.</p><p><b>RESULT</b>Baifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.</p><p><b>CONCLUSION</b>This method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.</p>

Experimental studies on acute toxicity of Achyranthes Aspera / 国际中医中药杂志

Objective To observe the acute toxicity of local achyranthes aspera, and its injury to major internal organs. Methods Estimated the dose before the experiment, then selected 40 mice and divided them into five groups randomly, namely 4 dose-groups and one control group. The doses used in the dose-groups were 400 g/kg, 300 g/kg, 225 g/kg and 169 g/kg respectively. Observed the symptoms and the death for successive 7 days and calculated LD (50)with statistic methods. Dissected the dead mice and observed the lesion of organs. Results The LD50 of acute toxicity test of achyranthes aspera was 309.21 g/kg, Sx=0.0359 g/kg. 95% of the limit of trust was 309.14～309.28 g/kg. According to the acute toxic standard, it belonged to non-toxicity. The specimen revealed that large dose administration caused coagulation of blood in both liver and spleen. Conclusion Local achyranthes aspera had little toxicity. Large dose administration affected heart function of mice.

Effects of isokinetic rehabilitation system to muscle strength training following knee joints injuries: Two-case analysis / 中国组织工程研究

BACKGROUND: Nowadays, more and more people regard the isokinetic training as a method for strength training and rehabilitation of muscle injuries of athletes. But it is rare in study of rehabilitation following joint injury via isokinetic training. OBJECTIVE: To explore the advantage of isokinetic training in rehabilitation, further more, to provide the scientific basis to rehabilitation of muscle injuries. DESIGN, TIME AND SETTING: The contrast observation was performed at the Laboratory of Institute of Physical Education, Hebei Normal University between March and May 2008. PARTICIPANTS: Two female college basketball athletes whose knee joints were injured. METHODS: The Kinitech isokinetic testing system (Kylingk, Australia) was used to test the flexion and extension muscles of knee joint of 2 athletes and contrasted the values of flexion and extension muscle after rehabilitation training, damage side and healthy side. All data were analyzed to observe the effects of strength training. MAIN OUTCOME MEASURES: Peak torque (PT) values of flexor and extensor in knee joints. RESULTS: After 8 weeks isokinetic training, the strength index of two subjects had increased in certain degree both at the 60 (?)/s and 240 (?)/s. The PT values showed that the increasing range of flexor was greater than that of extensor in the 1st subject, but the circumstance was opposite in the 2nd subject. The index of BP, STW, and AP was greatly increased, as well as work fatigue. CONCLUSION: It is helpful to increase the muscle strength and promote the rehabilitation via isokinetic training system. The isokinetic strength training has the positive effects on rehabilitation of muscle injuries.

Effects of endothelin-1 on the expressions of MMP-3 and TIMP-1 in human mesangial cells / 基础医学与临床

Objective To investigate the effects of endothelin-1(ET-1) on the expressions of matrix metalloproteinase-3(MMP-3) and tissue inhibitor of metalloproteinase-1(TIMP-1) in human mesangial cells(MCs).Methods Cultured human MCs were exposed to ET-1,the expressions of MMP-3 and TIMP-1 proteins were measured by ELISA.Results The expressions of MMP-3 and TIMP-1 in MCs were enhanced by ET-1,but the ratio of MMP-3/TIMP-1 decreased in a dose-dependent and time-dependent manner.Conclusion ET-1 may decreased the ratio of MMP-3/TIMP-1 in MCs,which then contribute to the inhibition of ECM degradation in glomerulus.

Preparation and dissolution of Wurenchun Dispersible Tablet / 中成药

AIM:To prepare Wurenchun Dispersible Tablet(Fructus schisandrae Chinensis)and investigate the dissolution of it.METHODS:The optimal formulation of Wurenchun Dispersible Tablets was established by the single factor experiments.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was determined by HPLC.RESULTS:Wurenchun Dispersible Tablets prepared by the mixture of 50 g extraction and 125 g CaHPO_4 plus excipent(MCC∶CMS-Na 3∶1)could disintegrate and disperse well within 3 min in(20?1)℃ water.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was higher than 80% in 15 min.CONCLUSION:Wurenchun Dispersible Tablets were stable,dissolved fast and completely.The preparation could promote the dissolution of total lignans.