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AIM: To guide the multiple imputation of missing data in clinical longitudinal studies and its sensitivity analyses, and highlight the importance of sensitivity analyses by taking the clinical trial of Qizhitongluo Capsule in treating ischemic stroke as an example. METHODS: To implement PROC MI process in SAS to perform multiple imputation and its sensitivity analysis. RESULTS: In the example, after multiple imputation, improvements in lower limb motor scores of the Qizhitongluo group were greater than those of the placebo group (all P<0.01), and the results of two sensitivity analyses under "missing not at random" were consistent with those under "missing at random". CONCLUSION: Multiple imputations combined with sensitivity analyses can ensure a robust result. It is recommended that clinical researchers perform sensitivity analyses after filling missing data.
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Objective To explore the use of non-invasive technique for early detection of atherosclerosis in health checkups. Methods A total of 46 824 healthy normotensive volunteers, including 24 879 men and 21 945 women, from 27 physical examination centers were reviewed for their clinical data. Pulse wave velocity (PWV) by age was calculated,and related factors of PWV were analyzed. Results Reference range of PWV was increased with age. Detectable rate of abnormal PWV in men < 60 years was significantly higher than women (P<0.05). Detectable rate of PWV of > 1400 cm/s, > 1700 cm/s or > 2000 cm/s were 34. 5% ,7. 5% and 1.9% Respectively. Correlation analysis showed that PWV was closely related with age and mean systolic blood pressure ( P < 0. 05 ). Conclusion Non-invasive technique for early detection of atherosclerosis and PWV should be useful in physical examinations and cardiovascular disease screening.
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Objective To study the inhibiting effects of phellinus igniarius extractive (PLE) on tumor growth of tumor-bearing mice in vivo, and the direct inhibiting action to human tumor in vitro. Methods ①Models of hepatoma,sarcoma, lung cancer in mice were established. Experiments in studying the effect of PLE on the above three animal models were repeated for three times. ②The suppression of cellular growth of human tumor was detected by MTT assay. Results PLE (250.500 and 1000 mg/kg, ig, d1 to d11) could inhibit the growth of H22 tumor cells in mice at the inhibitory rate of 57%,63% and 46%,respectively; slow down the growth of S180 tumor cells in mice at the inhibitory rate of 27.9%, 75%, and 31.2%,respectively; suppress the growth of Lewis lung cancer in mice at the inhibitory rate of 57.1%, 54.5%, 45.9%; and hold back the proliferation of MCF7 cells and OVCA2780 cells (P＜0.01). Conclusion PLE (250.500 and 1000 mg/kg, ig, d1 to d11)could inhibit the growth of H22 tumor cells, S 180 tumor cells, and Lewis lung cancer in mice. PLE could significantly reduce the proliferation of MCF7 cells and OVCA2780 cells (P＜0.05).