RESUMO
AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.
RESUMO
OBJECTIVE:To study the enhancing effects of azone at different concentrations on percutaneous permeation of compound tinidazole gel and to find out the best concentration.METHODS:Using vertical Franz-type diffusion cell and dif?ferent concentrations of azone as enhancer,the concentrations of tinidazole,chloramphenicol and salicylic acid in permeation solution were measured by RP-HPLC,then cumulative permeation quantity(Q)was calculated.RESULTS&CONCLUSION:Azone at different concentrations could promote the permeability of3ingredients of compound tinidazole gel,and2%azone had the most obvious permeation-enhancing effect.
RESUMO
AIM: To establish a HPLC method for the determination of tanshinone ⅡA in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C 18 (5 ?m, 250 mm?4.6 mm), protective column:(5 ?m, 12.50 mm?4.6 mm); mobile phase: methnaol-ultrapure water (80∶20); detective wavelength was at 270 nm; temperature was at 30 ?C ; the flow rate was about 1.0 mL?min -1 . RESULTS: The standard curve showed a good linearity within the range of 1?10 -2 -0.16 ?g, r=0.999 5. The average recovery was 99.73% and RSD was 2.91% . CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.
RESUMO
OBJECTIVE:To establish the quality control method of angelica capsule.METHODS:The chief components of the preparation,radix angelica sinensis,radix salvia miltiorrhizae,rhizoma corydalis,radix astragali,which were identified by TLC;the content of ferulic acid in angelica and tanshinoneⅡ A in Salvia were determined by HPLC method.RESULTS:The TLC(thin layer chromatogram)spots were clear;the linearity of ferulic acid was within the range of0.00255?g～0.0816?g(r=0.9997)and the linearity of tanshinoneⅡ A was within the range of0.01?g～0.06?g(r=0.9995).CONCLUSION:Th_ is method is simple and highly reproductive,which can be used for the quality control of preparation.