Clinical progress of the research an circulating tumor cells in colorectal cancer / 中国癌症杂志

Colorectal cancer is one of the most common malignant tumors. The majority of the patients die of metastases and recurrences after surgery. Circulating tumor cells (CTC) exist in the patients' peripheral blood, which can be considered as the crucial step in the metastasis cascade, thus being considered as the most valuable prognostic indicator. This paper summarized the clinical research works about CTC in eolorectal cancer during recent years.

Aberrant methylation of secreted frizzled-related protein genes in tissues of colorectal cancer and cancer cell lines / 中华消化杂志

Objective To investigate the association of promoter hypermethylation of secreted frizzled-related proteins (SFRPs) in patients with colorectal cancer. Methods The promoter hypermethylation of SFRPs in 20 sporadic colorectal cancer tissues and adjacent mucosa were detected by methylation-specific PCR. The amplified DNA was subcloned into the T-A cloning vector and sequenced. Two colorectal cancer cell lines (HCT116 and SW480) were treated with 5-aza-2' deoxycytidine for demethylation. The promoter hypermethylation and protein expression of SFRPs in colorectal cancer cell lines were detected by methylation-specific PCR and Western blotting. Results It was demonstrated that the hypermethylation of SFRP 1, 2, 4 or 5 was 19/20,17/20,3/20 or 13/20in cancer tissues, respectively, whereas it was 12/20, 8/12, 1/20 or 7/20 in adjacent mucosa,respectively. SFRP 1, 2 or 5 methylation was more frequently found in cancer tissue than in adjacent mucosa (P～0.05). Methylation of SFRP 1, 2, 4 and 5 were found in HCT116 cell line, but only SFRP1 and SFRP2 were found in SW480 cell line. There was a negative correlation between protein expression and methylation of SFRPs. The Western blotting revealed that SFRP protein re-expressedafter it treated with 5-aza-2' deoxyeytidine. Conclusion Methylation of SFRP 1, 2 or 5 gene is associated with the evolution of eolorectal cancer, and is closely related to silencing expression.

Clinical progress of the research on circulating tumor cells in colorectal cancer / 中国癌症杂志

Colorectal cancer is one of the most common malignant tumors. The majority of the patients die of metastases and recurrences after surgery. Circulating tumor cells (CTC) exist in the patients’ peripheral blood, which can be considered as the crucial step in the metastasis cascade, thus being considered as the most valuable prognostic indicator. This paper summarized the clinical research works about CTC in colorectal cancer during recent years.