RESUMO
A 48-year-old male was suffered from slowly progressive dysuria over twenty years. Although multiple urethral dilatation was treated, the effect was unsatisfactory. He was diagnosed with penile lichen sclerosus related to urethral stricture due to the lichenification of glans penis, the stenosis of urethral meatus, and the long anterior urethral stricture shown by urethrography. Finally, the patient underwent an enlarged urethroplasty with lingual mucosal graft (17 cm in length), and obtained a good outcome. During the two-year postoperative follow-up, the patient maintained a satisfactory urination without any complication.
RESUMO
Objective To investigate the role of MMPs expression in the invasion and metastasis of bladder cancer.Methods 50 patients with bladder cancer were selected as observation group .30 healthy people were selected as control group.ELISA method was used to detect the serum contents of MMP -2 and MMP -9.The distribution and expression of MMP -2 and MMP-9 were detected by immunohistochemical SP method .The results of the two groups were compared .Results The serum levels of MMP-2 and MMP-9 between bladder cancer with lymph node metastasis group and control group had statistically significant differences (t =7.532,6.358,all P <0.05 ) , and the differences between bladder cancer with lymph node metastasis group and bladder cancer without lymph node metastasis group were statistically significant (t =5.486,6.225,all P <0.05),and the differences between bladder cancer without lymph node metastasis group and normal control group were statistically significant (t=9.687,8.425,all P<0.05).The expression of MMP -2 and MMP-9 in bladder epithelial tissues of normal control group was negative , MMP-2 and MMP-9 expression in tumor cells and stromal cells of 50 patients with bladder cancer metastasis was positive ,mainly expressed in tumor cells ,MMP-2 positive expression in 37 patients, MMP-9 positive expression in 49 patients.With the increase of tumor grade and lymph node metastasis , the expression of MMP-2 and MMP-9 increased obviously,the differences were statistically significant (χ2 =9.268, 11.258,8.412,6.354,all P<0.05).MMP-2 and MMP-9 levels were associated with tumor histological grade and lymph node metastasis in bladder cancer cell metastasis (χ2 =11.742,P<0.05).Conclusion Serum contents and expression of MMP-2 and MMP-9 are closely related with invasion and metastasis of bladder cancer ,which can be used as objective indicators that estimate the prognosis of bladder transitional cell carcinoma .
RESUMO
asty without increasing morbidity, especially for slim patients or patients with a large renal pelvis.
RESUMO
Objective To investigate the effect of the nuclear factor (NF)-κBp65 antisense oligonucleotide (ASODN) on NF-κB activity and expression of interleukin(IL)-6 in hepatic stellate cells (HSC). Methods The HSC were separated from rats and cultured. The toxicity of NF-κBp65 ASODN on HSC were detected by Trypan blue exclusion staining and the NF-κB activity was determined by EMSA. The expressions of IL-6 mRNA and protein were meaured by RT-PCR and ELISA, respectively. Results In vitro, no toxicity of ASODN on HSC was observed at the concentrations of 0. 001 to 1.0 μmol/L. NF-κB activity was increased after stimulating HSC with tumor necrosis factor (TNF)α, whereas it was weakened in a dose dependent manner when HSC were cultured with ASODN (concentration from 0. 001 to 1.0 μmol/L). At the same time, the expressions of IL-6 mRNA and protein induced by TNFα were decreased after transfected with ASODN at concentrations of 0.001- 1. 0 μmol/L in a dose dependent manner. Conclusion ASODN may specifically inhibit either the activiy of NF-κB or expression of IL-6, which provides the theoretical basis that ASODN may use to treat fibrosis of the liver.