Chloroplast genomic characterization and phylogenetic analysis of Castanopsis hystrix / 生物工程学报

The structure and size of the chloroplast genome of Castanopsis hystrix was determined by Illumina HiSeq 2500 sequencing platform to understand the difference between C. hystrix and the chloroplast genome of the same genus, and the evolutionary position of C. hystrix in the genus, so as to facilitate species identification, genetic diversity analysis and resource conservation of the genus. Bioinformatics analysis was used to perform sequence assembly, annotation and characteristic analysis. R, Python, MISA, CodonW and MEGA 6 bioinformatics software were used to analyze the genome structure and number, codon bias, sequence repeats, simple sequence repeat (SSR) loci and phylogeny. The genome size of C. hystrix chloroplast was 153 754 bp, showing tetrad structure. A total of 130 genes were identified, including 85 coding genes, 37 tRNA genes and 8 rRNA genes. According to codon bias analysis, the average number of effective codons was 55.5, indicating that the codons were highly random and low in bias. Forty-five repeats and 111 SSR loci were detected by SSR and long repeat fragment analysis. Compared with the related species, chloroplast genome sequences were highly conserved, especially the protein coding sequences. Phylogenetic analysis showed that C. hystrix is closely related to the Hainanese cone. In summary, we obtained the basic information and phylogenetic position of the chloroplast genome of red cone, which will provide a preliminary basis for species identification, genetic diversity of natural populations and functional genomics research of C. hystrix.

Protective effects of shaoganduogan on hepatocyte mitochondria in subacute liver injury rat induced by carbon tetrachloride / 中国中药杂志

<p><b>OBJECTIVE</b>To study the protective effect of Shaoganduogan (SGDG) on serum transaminase, liver pathology and hepatocyte mitochondria in rat with subacute liver injury induced by carbon tetrachloride.</p><p><b>METHOD</b>Subacute liver injury of rats were induced by carbon tetrachloride, and cured by different doses of SGDG through intragastric administration. The activity of serum ALT, AST, liver pathology and ultrastructure, activity of ATPase, SOD and content of MDA of hepatocyte mitochondria were observed.</p><p><b>RESULT</b>SGDG can remarkably reduce the transaminase, alleviate the degeneration and necrosis of liver cells ,enhance activity of Na+ -K+ ATPase, Ca2+ ATPase, SOD, reduce content of MDA of mitochondria, alleviate ultrastructure change of mitochondria, reduce section area, perimeter equivalent diameter and average optical density perimeter of liver cells.</p><p><b>CONCLUSION</b>SGDG has obvious effect of liver protection, the mechanisms are related with alleviating mitochondria injury.</p>

Therapeutical Effect of Zangfukang Suppository on Experimental Vaginitis in Rats / 中国药房

OBJECTIVE: To evaluate the therapeutic effect of Zangfukong suppository on the experimental vaginitis.METHODS:The experimental rats were equally assigned to 6 groups: normal control group,model control group,gyno-daktarin(0.12 g?kg-1) group,three Zangfukang groups(0.33 g,0.17,and 0.08 crude drug? kg-1).Candida albicans solution was injected into the vigina of the estrous rats to establish colpomycosis model.The effect of Zangfukang suppository on the infection rate of candida albicans was observed.The bacterial vaginitis model was established by injecting the mixture bacteria of Neisseria gonorrhoeae,staphylococcus aureus and escherichia coli into the vagina of rats,and the effect of Zangfukang suppository on the gonococcal infection rate.RESULTS: After local application of Zangfukang suppository for 7 consecutive days for treatment of colpitis mycotica of rats,the infection rates of candida albicans were significantly lower in medium-dose and high-dose groups than in the model control group(P