RESUMO
AIM:To explore the recombined human proinsulin gene containing glucose reaction element(GLRE) expression in transfected CBRH7919 cells.METHODS: The packaged retrovirus encoding genetically modified human proinsulin PLXSN-(GLRE)3-BP-1MpINS3 and PLXSN-(GLRE)3-BP-1MpINS2 were transfected into CBRH7919 cells.Insulin values in cells after transient and steady expression screened by G418 at different glucose levels were detected.Chromosome DNA was isolated from transfected and untransfected cells and polymerase chain reaction(PCR) was performed.PCR products were analyzed by electrophoresis.RESULTS: 38 h after transfection,at the glucose levels of 0-25 mmol/L,the levels of insulin produced by cells including PLXSN-(GLRE)3-BP-1MpINS3 were(3.57?0.21)U/L,(5.30?0.20)U/L,(16.27?0.87)U/L,(23.23?1.12)U/L,respectively(P