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【Objective】 To analyze the current situation of human parvovirus B19 infection in blood donors in different regions of China, so as to provide basis for formulating reasonable screening programs of B19 virus for blood donors in various cities and regions. 【Methods】 The literatures related to human parvovirus B19 infection in whole blood and plasma donors published from 1998 to 2021 were searched in the database, and meta-analysis of literatures that satisfied the inclusion criteria was conducted by R4.1.0 software. 【Results】 A total of 35 literatures were obtained, 20 literatures involving 56 846 blood donor samples and 8 literatures involving 1 608 pooled raw plasma samples were subjected to Meta analysis of the positive rates of B19 DNA; 17 literatures involving 12 308 blood sample were subjected to the Meta analysis of the positive rate of B19 IgG antibody.The positive rates of B19 DNA in blood donors(I2=96%, τ2=0.026 0, P<0.01)and pooled raw plasma (I2=98%, τ2=0.124 5, P<0.01), as well as the positive rate of B19 IgG antibod (I2=98%, τ 2 =0.021 0, P < 0.01) presented significant heterogeneity between regions. The combined positive rate of B19 DNA was estimated to be 2.0% (95%-CI: 0.007~0.039), that of pooled raw plasma for production was 66.6% (95%-CI: 0.476~0.832), and that of B19 IgG antibody was 30.2% (95%-CI: 0.246~0.357). 【Conclusion】 Low HPV B19 infection rate and high positive rate of IgG antibody were found in blood donors. Therefore, the risk of B19 virus infection due to blood transfusion is low, but B19 infections in blood donors varied significantly between regions. Domestic cities and regions should reasonably evaluate their own B19 virus infection status to formulate appropriate B19 virus screening programs for blood donors, so as to reduce blood transfusion transmitted risk of B19 virus. In addition, the infection rate of B19 virus in pooled plasma for production is somewhat high. Recipients should be screened for B19 virus antibodies, and appropriate blood transfusion schemes should be formulated for blood recipients lacking neutralizing B19 IgG antibodies to reduce the exposure of B19 virus.
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Objective To observe the effect of Huoxue-Yixin decoction combined with atorvastatin on NT-pro and QTd in patients with chronic heart failure (CHF). Methods A total of 120 patients with chronic heart failure in our hospital from December 2016 to June 2017 were enrolled in this study. The subjects were randomly divided into the control group (n=60) and the treatment group (n=60). The control group were treated with conventional treatment,and the treatment group were treated with Huoxue-Yixin decoction combined with atorvastatin. The two groups were treated for 30 days. The NT-ProBNP, QTd, QTcd and LVESD, LVEDD, LVEF of the two groups before and after treatment were compared.Results The QTd at 2 d(45.6 ± 8.9 ms vs.64.0 ± 18.2 ms,t=-7.029),14 d(44.3 ± 6.8 ms vs.55.7 ± 12.8 ms,t=-6.092)in the experiment group were significantly lower than those of control group(P<0.01).The level of QTcd at 2 d(50.8 ± 10.1 ms vs.71.2 ± 19.8 ms,t=7.109), 14 d(48.1 ± 9.7 ms vs.63.1 ± 13.1 ms,t=7.128)of the experiment group were significantly lower than those of control group(P<0.01).After treatment,the NT-ProBNP(494.58 ± 331.61 ng/L vs.594.71 ± 382.65 ng/L,t=-1.532), LVESD(40.23 ± 3.13 mm vs.49.72 ± 3.99 mm,t=-14.495)and LVEDD(58.22 ± 3.30 mm vs.65.11 ± 3.95 mm, t=-10.720)of the treatment group were significantly lower than those of the control group(P<0.01).The LVEF (69.82% ± 4.72% vs. 50.34% ± 4.02%, t=-10.720) of the treatment group was significantly higher than the control group(P<0.01).Conclusions The Huoxue-Yixin decoction combined with atorvastatin can reduce the level of NT-ProBNP, QTd and QTcd in patients with CHF, can significantly improve cardiac function.
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Objective: To explore influence of sleep deprivation (SDP) on serum interleukin-1β (IL-1β) level in rats with coronary atherosclerosis (CAS). Methods: A total of 96 healthy SD rats were randomly and equally divided into normal control group, CAS group, SDP group and SDP + CAS group according to number table; rats in CAS group and SDP + CAS group were fed with high cholesterol diet to establish CAS animal model; SDP group and SDP + CAS group received SDP through small platform with water environment. Radioimmunoassay was used to measure serum concentration of IL-1β; and serum concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were measured respectively; above indexes were compared among four groups. Results: (1) Compared with serum IL-1β level of normal control group, there were significant increase in SDP group, CAS group and SDP + CAS group[(13.33±3.20) ng/L vs. (14.40±4.41) ng/L vs. (22.21±5.94) ng/L vs. (30.17±7.45) ng/L] (P<0.05～<0.01); (2) Levels of TC, TG and LDL-C in CAS group and SDP + CAS group were significantly higher, level of HDL were significantly less than those of normal control group and SDP group (P<0.05～<0.01), levels of above indexes in SDP + CAS group were significantly higher or less than those of CAS group (P<0.05 all). Conclusion: Sleep deprivation can significantly heighten serum interleukin-1β level and total cholesterol, triglyceride, low density lipoprotein-cholesterol levels, decrease HDL level in rats with coronary atherosclerosis or sleep deprivation.
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Objective:To evaluate whether oral administration of heat shock protein60(HSP60) could induce immune tolerance and its effect on the progression of aortic atherosclerotic plaque in hypercholesterolemic apolipoprotein(apo) E-/-mice.Methods:8 week-old mice were divided into two groups that were orally administrated PBS plus HSP60 and only PBS separately for 5 days,and then a high-cholesterol diet was started 2 days after the last treatment for 12 weeks.Pathological analysis of plaque was performed,and percentage of CD4+CD25+regulatory T cells in the splenocytes was analyzed,proliferation response of the splenocytes to HSP60 was detected and cytokines in the superanant were measured.Results:Compared with control animals,oral tolerance to HSP60 resulted in a significant decrease of atherosclerotic plaques in size.Tolerance to HSP60 induced a significant increase of CD4+CD25+ cells in the spleen.Specific proliferation response of splenocytes to HSP60 was significantly suppressed and TGF-? in the superanant increased while IFN-? decreased significantly.Conclusion:HSP60 specific tolerance can be induced via oral administration of HSP60 which in turn attenuates progression of plaque.This provides a new immunologic approach for treatment of atherosclerosis.