Development and present situation of breast milk bank / 国际儿科学杂志

Foreign Breast milk Banks have developed for more than 100 years in foreign countries.They have not only established the corresponding the Human Milk Banking Association of North America and the European Breast Milk banks Association accordingly,but also established a mature breast milk banks management guideline.However,the research on cognition and technology of breast milk bank in China is still in the stage of exploration and needs further development.The desire for breast milk donation and acceptance of d onor human milk in puerpera are limited.At present,as more and more attention were paid to breast milk feeding,the current situation cannot meet the needs of medical development.We need a formal management guideline to guide the development of breast milk bank.In this article,we introduce the concept and importance of breasffeeding,the necessity and feasibility of establishing breast milk banks,the development prospects of the breast milk bank,for reviewing a systematic thinking of the cognitive formation of breast milk bank,and other aspects to form a system review of the knowledge of breast milk bank.We aim to let puerpera have a better understanding of breast milk bank,eliminate the concerns about donating breast milk and increase the breast milk donation rate,thus the premature babies and newborns with diseases will benefit from the construction of the breast milk bank.

The impact of meteorological and environmental conditions on the spread of COVID-19 / 公共卫生与预防医学

Objective To understand the impact of meteorological and environmental conditions on the spread of COVID-19, and to provide a scientific basis for prediction, prevention and control of COVID-19. Methods The number of confirmed cases, population density and meteorological data of all cities in Hubei Province during COVID-19 outbreak period were collected. The relationship between COVID-19 infection rate and climatic factors were analyzed using Excel and SPSS software. Results The infection rate of COVID-19 had the greatest correlation with population density. The infection rate of COVID-19 showed a very significant power-law relationship with the number of the hot days before COVID-19 outbreak, and it also had a good correlation with the high temperature accumulation, the rank of hot days and the drought intensity. Conclusion The adverse effect of high temperature and heat wave on the human body had an impact on the spread of COVID-19. The ability of people in different areas to resist high temperature was different because of different climates. Drought may affect the spread of COVID-19 by destroying the ecological environment.

Neuronavigator-assisted microsurgical resection of glioma located in cerebral functional areas / 中南大学学报(医学版)

Objective:To evaluate value of neuronavigator-assisted microsurgery of glioma located in cerebral functional areas.Methods:Patients with glioma located in cerebral functional areas were underwent operation in Xiangya Hospital.Of 64 patients,34 patients were performed neuronavigator-assisted microsurgery,and 30 were underwent routine surgical operation.Results:The neuronavigator-assisted microsurgery group showed high complete resection rate with low neurological deficit and cerebral edema compared with the routine surgical group (P＜0.05).Conclusion:Neuronavigator-assisted microsurgery is effective and characterized by accurate location,personalized operative incision design,and higher rate of tumor resection.

Differential expression of DJ-1 and HSP27 in invasive and non-invasive pituitary adenomas / 中南大学学报(医学版)

OBJECTIVE@#To explore whether oncogenes DJ-1 and HSP27 are associated with invasiveness of human pituitary adenoma.@*METHODS@#Total proteins were extracted from samples of 20 invasive and 20 non-invasive pituitary adenomas and the expression of DJ-1 and HSP27 was analyzed by Western blot. The correlation of DJ-1and HSP27 with the invasiveness of pituitary adenoma was analyzed.@*RESULTS@#The strong positive rates of DJ-1 and HSP27 in the 20 invasive pituitary adenoma were 70% (14/20) and 80% (16/20), respectively. The invasive group had significantly higher expression of DJ-1 and HSP27 proteins than the noninvasive group [10% (2/20), 10% (2/20), respectively]. There was a positive correlation between the expression of DJ-1, HSP27 proteins and the invasiveness of pituitary adenoma as judged by the Spearman rank correlation test (P<0.05).@*CONCLUSION@#The proliferative activity and abnormal expression of oncogenes DJ-1 and HSP27 may play a significant role in tumorigenesis and progression of pituitary adenoma. There was a significant correlation between the expression of DJ-1 and HSP27 and the invasiveness of pituitary adenoma.

Efficacy of subarachnoid space protection in intracranial operation: a report of 156 cases / 中南大学学报(医学版)

OBJECTIVE@#To determine the efficacy of subarachnoid space protection in intracranial operation.@*METHODS@#Data collected from 156 consecutive cranial operations, in which subarachnoid space protective technology was prophylacticly used, were analyzed.@*RESULTS@#Fourteen patients had a postoperative fever for more than 1 week and 16 patients who required lumbar puncture to release blood contaminated cerebro-spinal fluid (CSF) or exclude meningitis. All except 3 patients were discharged as expected. No patients had symptomatic vasospasm and hydrocephalus.@*CONCLUSION@#The subarachnoid space protective technology has good effect on preventing postoperative fever and improving the outcome of patients.

Effect of epidural drainage and dural tenting suture on epidural hematoma in 145 cases of craniotomy / 中南大学学报(医学版)

Objective To evaluate the efficacy of dural tenting suture and epidural drainage in craniotomy. Methods In 145 cases of intracranial lesions, dural tenting suture and epidural drainage were performed to prevent epidural hematoma. Results Postoperative computed tomography (CT) showed no epidural hematoma required surgery in both groups. Conclusion Both dural tenting suture and epidural drainage are effective in preventing epidural hematoma. Hemostasis is the key step. Dural tenting suture without epidural drainage relieves psychological stress. It decreases the risk of intracranial infection and avoids some unusual complications.

Differential proteomic analysis of thalamus in rats with diffuse axonal injury / 中南大学学报(医学版)

OBJECTIVE@#To examine the differential expression of protein of thalamus in rats with diffuse axonal injury.@*METHODS@#Twenty-five rats were randomly divided into a normal group (n=10) and a trauma group (n=15). Total proteins of brain trauma tissue and normal brain tissue were extracted separately, and then proteins were separated by two dimensional gel electrophoresis and stained with Coomassie brilliant blue. The differentially expressed protein spots were identified with biospectrometry. Images were analyzed by PDQuest 7.0.@*RESULTS@#The distribution of protein spots in the trauma group was similar to that of the normal group, the matching rate was 95%, and the repeatability was good. Proteins were mainly displayed at pI 3-8, with relative molecular mass 14.4-75.0 kD. Compared with the normal group, 16 spots of proteins increased and 18 spots of proteins decreased in the trauma group.@*CONCLUSION@#There is some difference in protein expression between the normal group and the trauma group. Brain trauma may lead to changes of proteins in the thalamus.

Establishment of differential expression profiles from invasive and non-invasive pituitary adenomas / 中南大学学报(医学版)

Objective To establish high resolution, reproducible 2-dimensional electrophoresis (2-DE) profiles of invasive and non-invasive pituitary adenoma tissues and to identify differentially ex-pressed proteins between the invasive and non-invasive tissues. Methods The proteome from invasive and non-invasive pituitary adenomas tissues was dissected and analyzed by: (1) immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis, (2) silver staining, (3) imageMaster 2-D software analysis, (4) peptide mass fingerprint based (PMS) on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and (5) database comparison. Results High-resolution 2-D patterns of invasive and non-invasive pituitary adenoma tissues were successfully produced and re-peated 3 times for each sample. An average of 1080±24 and 1035±28 spots were detected for invasive and non-invasive pituitary adenoma tissues, respectively. Additionally, 975±45 and 918±56 spots were found to have an average matching rate of 90.3% and 88.7% for invasive and non-invasive tissues, re-spectively. The spot positional deviation was (1.563±0.259) mm for IEF and (1.088±0.206) mm for SDS-PAGE. A total of 99 spots of differential expression were matched between the invasive and non-in-vasive pituitary adenoma tissues. Thirty differential proteins, some of which were involved in the regula-tion of cells cycle and signal transduction, were initially characterized by PMS. Conclusion The acquisi-tion of well-resolved and reproducible 2-D patterns of invasive and non-invasive pituitary adenoma tissues and the identification of differentially expressed proteins provides a proteome database for invasive pituita-ry adenomas.

mRNA expression of neuroglobin in thalamus of DAI rats / 中国医师杂志

Objective To study mRNA expression of neuroglobin (NGB) in thalamus of DAI rats and explore the relation between NGB and heed injury. Method 46 male SD rats were randomly divided into four groups. The DAI model was established according to modi-fied Mamamu method. The mRNA expression of NGB in thalamus at different time point after injury was detected by RT-PCR method. All data were analyzed with statistical software. Result The mRNA expression of NGB in thalamus increased obviously from 30 minutes after in-jury while decreased at the 24th hour after injury. But the expression increased again and reached peak at the 48th hour after injury. Conclu-sion The mRNA expression of NGB in thalamus increased after DM. Those changes suggested NGB might participate in the response mech-anism of stress, secondary ischemia and hypoxic brain injury after neuron injury.

Ventriculoperitoneal shunt for communicating hydrocephalus / 中国医师进修杂志

Objective To summarize the experience of the ventriculoperitoneal shunt (VPS) in communicating hydrocephalus and its complications. Methods The clinical features, operative techniques and outcome of 100 patients with hydrocephalus were analysed retrospectively. Results Ninety-five (95%) cases had a good result. Postoperative complications were found in 6(6%) cases including shunt apparatus blockage (4 cases) and shunt infection (2 cases). All the cases improved after taking the corresponding measures. Conclusion VPS is the most common shunt style for communicating hydrocephalus. The shunt apparatus blockage and infection are common postoperative complications. Intraoperative aseptic technique, the minimally invasive procedure, and the optimal placement of shunt tube may play an important role in improving the outcome of cerebrospinal fluid shunting surgery for communicating hydrocephalus.

Effect of Bubble Bath Coordinating with Functional Training on the Children with Spastic Cerebral Palsy / 中国康复理论与实践

@#ObjectiveTo observe the effect of bubble bath coordinating with functional training on the children with spastic cerebral palsy.Methods210 children with spastic cerebral palsy were randomly divided into the treatment group (n=118) and control group (n=92). All children were treated with functional training, acupuncture and other physical therapies. Children in the treatment group were added with bubble bath one time every day, twenty days as one course. Muscular tension was evaluated with modified Ashworth Scale and range of motion was measured before and after treatment.ResultsIn the treatment group, 55 cases were significant, 53 cases were effective, 10 cases were invalid, the total effective rate was 91.5%; in the control group, 26 cases were significant, 46 cases were effective, 20 cases were invalid, the total effective was 78.2%. There was a significant difference between the total effective rates of two groups (P<0.05).ConclusionBubble bath can significantly decrease the muscular tension of children with spastic cerebral palsy.