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Biomedical and Environmental Sciences ; (12): 786-793, 2014.
Artigo em Inglês | WPRIM | ID: wpr-270539

RESUMO

<p><b>OBJECTIVE</b>To assess the effect of atorvastatin on lipopolysaccharide (LPS)-induced TNF-α production in RAW264.7 macrophages.</p><p><b>METHODS</b>RAW264.7 macrophages were treated in different LPS concentrations or at different time points with or without atorvastatin. TNF-α level in supernatant was measured. Expressions of TNF-α mRNA and protein and heme oxygenase-1 (HO-1) were detected by ELISA, PCR, and Western blot, respectively. HO activity was assayed.</p><p><b>RESULTS</b>LPS significantly increased the TNF-α expression and secretion in a dose- and time-dependent manner. The HO-1 activity and HO-1 expression level were significantly higher after atorvastatin treatment than before atorvastatin treatment and attenuated by SB203580 and PD98059 but not by SP600125, suggesting that the ERK and p38 mitogen-activated protein kinase (MAPK) pathways participate in regulating the above-mentioned effects of atorvastatin. Moreover, the HO-1 activity suppressed by SnPP or the HO-1 expression inhibited by siRNA significantly attenuated the effect of atorvastatin on TNF-α expression and production in LPS-stimulated macrophages.</p><p><b>CONCLUSION</b>Atorvastatin can attenuate LPS-induced TNF-α expression and production by activating HO-1 via the ERK and p38 MAPK pathways, suggesting that atorvastatin can be used in treatment of inflammatory diseases such as sepsis, especially in those with atherosclerotic diseases.</p>


Assuntos
Animais , Camundongos , Adjuvantes Imunológicos , Farmacologia , Atorvastatina , Ativação Enzimática , Heme Oxigenase-1 , Genética , Metabolismo , Ácidos Heptanoicos , Farmacologia , Inibidores de Hidroximetilglutaril-CoA Redutases , Farmacologia , Lipopolissacarídeos , Farmacologia , Macrófagos , Proteínas de Membrana , Genética , Metabolismo , Pirróis , Farmacologia , Fator de Necrose Tumoral alfa , Metabolismo
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