Effects of electroacupuncture on expression of calmodulin in the hippocampus of rats with cerebral ischemia-reperfusion injury / 中国针灸

<p><b>OBJECTIVE</b>To investigate the protective effect of electroacupuncture (EA) on injured neurons and the signal transduction mechanism of calmodulin (CaM) in rats with cerebral ischemia-reperfusion injury (CIRI).</p><p><b>METHODS</b>A total of 25 SD rats were randomly divided into a sham-operation group, a model group, an EA. group, a TFP group and an EA+TFP group. The rat model of middle cerebral artery occlusion (MCAO) was established by the modified Longa thread occlusion method. The EA group was treated with EA at "Dazhui" (GV 14) and "Baihui" (GV 20) for 30 minutes. The TFP group was treated with lumbar intrathecal injection of Trinuoperazine (TFP) at a dose of 40 microL/kg, the inhibitor of CaM. The EA + TFP group was treated with EA combined with TFP, and the sham-operation group and the model group without any treatment. The neurology deficit score was evaluated by the Julio's neuroethology score methods in all rats, and the expression of CaM in cerebral hippocampus tissue was detected with immunohistochemical method in different intervention condition.</p><p><b>RESULTS</b>(1) In comparison with the model group of 6.90 +/- 1.66, the neuroethology score in the EA group of 14.50 +/- 1.08, the TFP group of 11.70 +/- 1.06 and the EA + TFP group of 14.30 +/- 1.06 were all significantly increased (all P < 0.01), while those still were all lower than the sham group of 17.60 +/- 0.52 (all P < 0.01), and the EA group was better than the TFP group (P < 0.01). (2) In comparison with the sham group of 0.080 +/- 0.045, the immune positive expression score of CaM protein in hippocampus in the model group of 1.680 +/- 0.268 was sig nificantly increased (P < 0.01). In comparison with the model group, the expression score of CaM protein in the EA group of 0.880 +/- 0.179, the TFP group of 0.720 +/- 0.179 and the EA + TFP group of 0.420 +/- 0.249 were all significantly reduced (all P < 0.01), and the expression score of CaM in the EA + TFP group was lower than that in the TFP group (P < 0.05).</p><p><b>CONCLUSION</b>EA can reduce the injury of cerebral neurons induced by CIRI in rats and promote the recovery, which may be related to its effect in regulating CaM signaling pathway after the ischemia-reperfusion injury.</p>

Survey of research on acupoints compatibility / 中国针灸

The research papers that meet the criteria of evidence-based medicine and randomized controlled trial were retrieved in Chinese journals data bases (CNKI knowledge network) from 1992 to 2009. Twenty-five papers indicate that acupoints compatibility rules are closely related to organism regional anatomy, nerve, the blood vessel and the endocrine gland; acupoints compatibility rules produce synergism, inhibit or antagonistic effect that affect the clinical effectiveness. The acupoints compatibility rules based on experimental researches are applied to clinic practice is the key to improve the acupuncture clinical effectiveness.

Clinical observation on mild-warm moxibustion for treatment of diabetic peripheral neuropathy / 中国针灸

<p><b>OBJECTIVE</b>To observe clinical therapeutic effect of mild-warm moxibustion on diabetic peripheral neuropathy (DPN) and to probe the mechanism.</p><p><b>METHODS</b>Sixty cases of DPN were randomly divided into a mild-warm moxibustion group, an acupuncture group and a medication group, 20 cases in each group. In the mild-warm moxibustion group and the acupuncture group, the same points, Shenshu (BL 23), Pishu (BL 20), Zusanli (ST 36), Yongquan (KI 1), etc. were selected; and the medication group were treated with Mecobalamin tablets. Their therapeutic effects and changes of fasting blood-glucose (FBG), glycosylated hematoglobin (GHB), hemorheological indexes, plasma endothelin (ET), nitric oxide (NO) and malondialdehyde (MDA) before and after treatment were investigated.</p><p><b>RESULTS</b>The total effective rate was 90.0%, FBG, GHB, hemorheological indexes, plasma ET, NO and MDA significantly improved in the mild-warm moxibustion group (P < 0.01), with no significant difference as compared with those in the acupuncture group (P > 0.05), but with a significant difference as compared with the medication group (P < 0.05).</p><p><b>CONCLUSION</b>Mild-warm moxibustion has definite therapeutic effect on diabetic peripheral neuropathy, which is better than that of Mecobalamin.</p>

Clinical experiences and thinking of acupuncture for treatment of some difficult and complicated eye's diseases / 中国针灸

The present paper introduces author's some successful experiences on acupuncture treatment of some difficult and complicated eye's diseases in recent years. The author has systematically studied on the law and acupoints of acupuncture for treatment of some difficult and complicated eye's diseases, and worked out therapeutic principles and selected acupoints by means of syndrome differentiation, treating both the principal and the second aspects of a disease, its symptoms and cause at the same time, selecting acupuncture points according to syndromes, combination of Chinese medicine with western medicine, combination of acupuncture with medicine, etc. to strengthen the body resistance and eliminate pathological factors, attaining very satisfactory clinical therapeutic effects.

Application of TC index location on Longa' s animal model of regional experimental cerebral ischemia and reperfusion / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the TC index location on a reform LONGA' s animal model of regional experimental cerebral ischemia and reperfusion.</p><p><b>METHODS</b>A total of 300 SD male rats were randomly divided into the pre-test group (n = 30), the original method group (n = 120, Longa' s method) and the reform method group [ n = 150, TC index location is defined by the length of rat incisor teeth root (T point) to the common carotid artery bifurcation (C point) ,the blood flow of a unilateral middle cerebral artery in the rats was reversibly blocked by the TC index location). The morphologic changes and the successful rate of the two methods to establish the rat model were compared.</p><p><b>RESULTS</b>The successful rate of TC index location modified model method was superior to that of original LONGA' s method,with significant difference in linear correlation analysis.</p><p><b>CONCLUSION</b>The reform LONGA' s animal model of regional experimental cerebral ischemia and reperfusion by the TC index location is a good method, and is worth applying in experimental study.</p>

Progress in researches on the effect of acupuncture in antagonizing oxygen stress / 中国结合医学杂志

Oxidation and free radicals participate in the pathological process of multiple diseases in organisms, and acupuncture shows good effect in antagonizing oxygen stress (OS). This article reviews the effect of acupuncture in antagonizing oxygen stress and the mechanism of its anti-free radical effect in various diseases. The authors hold that acupuncture not only has a chain-blocking effect, but also has preventive and repairing effects of anti-oxidation. And anti-OS action is one of the important mechanisms of acupuncture.