Therapeutic effect and timing of reteplase in patients with ST-segment elevation myocardial infarction / 心血管康复医学杂志

Objective:To observe the therapeutic effect and timing of reteplase in patients with ST-segment elevation myocardial infarction (STEMI) .Methods:A total of 106 STEMI patients were selected from Central Hospital of Wafangdian City of Liaoning Province , from Jan 2013 to Apr 2014 ,and all patients received thrombolytic treat-ment .According to the duration from onset to thrombolysis ,patients were divided into :group 1 with duration 0.05 all) .Conclusion:The thrombolytic recanalization rate is highest ,and the 30d all-cause mortality and incidence rate of adverse reactions are lowest for thrombolysis within 3 h from onset .

CR Chest Radiography and Image Features at Fever Clinics / 医疗卫生装备

Objective To discuss the image features of different types of pneumonia. Methods The method and flow for CR chest radiography at fever clinics were described. Results The method and flow for CR chest radiography at fever clinics were improved. Conclusion Method improvement is very significant.

The Progress of the Methods for Screening DifferentiallyExpressed Genes and Proteins / 生物化学与生物物理进展

Cloning and identification of differentially expressed genes or proteins is helpful not only for finding the functions of genes and proteins, but also for discovery of the mechanism of some diseases. Some methods have been developed for screening differentially expressed genes, such as differential display RT-PCR (DDRT PCR), subtractive hybridization (SH), DNA chip technique, and serial analysis of gene expression (SAGE). In subtractive hybridization, there have advanced three improved methods which include representational difference analysis (RDA), suppression subtractive hybridization (SSH), and full-length-gene-obtainable subtractive hybridization. For obtaining differentially expressed proteins, scientists have only two choices so far. One is two-dimentional gel electrophoresis. The other is phage display antibody repertoire library technique. Since all of the methods above have their own advantages and disadvantages, they should be used according to different needs.screening, differentially expressed genes, differentially expressed proteins

Improved PCR-based subtractive hybridization, a new strategy on cloning differential expression genes in apoptotic MCF-7 cells / 细胞与分子免疫学杂志

Aim To clone apoptosis-related genes from human MCF-7 breast cancer cells and to analyze the character of the method used in the process. Methods A poptotic cell model of MCF-7 cells was established with the apoptotic tumor cells induced by the all-trans-retinoic acid. The apoptotic gene was cloned from the model by improved PCR-based subtractive hybridization. Results 5 clones were identified to be related to apoptosis by reverse dot blot, 4 of them were known genes, and 3 were related to apoptosis. A novel gene， named apmcf-1, coded for 47 amino acid was identified. This gene was accepted by Genbank, the accession number was AF141882. Conclusion This improved PCR-based subtractive hybridization may be an efficient way in cloning differential expression gene.

Effects of Prevotella intermedia lipopolysaccharide on the gene expression in periodontal ligment cells / 实用口腔医学杂志

Objective:To study the gene expression in periodontal ligament cells (PDLCs) stimulated by Prevotella intermedia(Pi) lipopolysaccharide (LPS). Methods:PDLCs of passage 7 were treated with Pi LPS at 100 ?g/ml for 24 h, then gene express of the cells was examined by a gene chip of 512 genes. Results: Genes of Z46973,M62810,AF109632,U45879,X81882,M10051,D14520 and L36529 were downregulated.Conclusion:Some genes in PDLCs can be downregulated by Pi LPS.

Cloning of Human Interleukin-17 and Its Expression in E. coli / 中国肿瘤生物治疗杂志

Interleukin-17 (IL-17) is a newly found cytokine secreted by activated T lymphocytes. From preliminary study, IL-17 was found to play a role in the relationship between T lymphocytes and hematopoiesis, but many of its characteristics remain unknown up to now. To investigate its biological functions and related mechanisms, we cloned the complete coding region of human IL-17(hIL-17) from activated human peripheral blood mononuclear cells (PBMC) by RT-PCR and constructed an integrative vector pLCM182-hIL-17 for cloning, expressing and sequencing the hIL-17 gene. By DNA sequencing, the cloned hIL-17 is identical to the reptorted, and with temperature inducing, the hEL-17 was highly expressed in E. coli up to 30% of bacterial protein. The expressed hIL-17 protein could stimulate primary human fibrob-lasts to secrete IL-6 and GM-CSF after initial purification. This results indicate that the expressed hIL-17 has biological activity.