Transfection of adeno-associated virus encoding beta-nerve growth factor into endothelial progenitor cells / 中国组织工程研究

BACKGROUND:Nowadays, gene therapy has become a new trend for disease therapy and brought promise for some refractory diseases. Its key is to choose proper cel s, genes and vectors. OBJECTIVE:To use recombinant adeno-associated virus mediatedβ-nerve growth factor (β-NGF) to transfect rat bone marrow-derived endothelial progenitor cel s in vitro, and to investigate the effect ofβ-NGF expression on the proliferation of endothelial progenitor cel s. METHODS:The endothelial progenitor cel s were isolated, cultured and identified from the bone marrow of rats. Empty vector or recombinant adenovirus-associated virus containingβ-NGF gene was transferred into endothelial progenitor cel s. We examined the transfection efficiency by fluorescence expression of green fluorescent protein. Expression ofβ-NGF protein was detected using ELISA, and its effect on the proliferation of endothelial progenitor cel s was determined using MTT method. RESULTS AND CONCLUSION:Rat endothelial progenitor cel s were isolated and cultured successful y in vitro and were identified positive by the function of cel s and immunofluorescence staining. The endothelial progenitor cel s were infected directly by the recombinant adenovirus-associated virus containingβ-NGF gene with an efficiency of 65.3%.β-NGF protein was detected in the culture supernatant of transfected endothelial progenitor cel s, which reached a high level at 10 days after gene transfection. Furthermore, there was noβ-NGF protein in the blank and empty vector groups. After transfection, the proliferative ability of endothelial progenitor cel s was increased, which was significantly higher than the blank and empty vector groups (P0.05). These findings suggest that recombinant adenovirus-associated virus containingβ-NGF gene can be successful y transferred into rat bone marrow-derived endothelial progenitor cel s and promote the proliferation of endothelial progenitor cel s.

STUDY ON IMPROVED McAb-ELISA IN DETECTING BLOOD STAGE P.VIVAX ANTIGEN / 中国血吸虫病防治杂志

This paper reports an improved McAb-ELISA test in detecting blood stage P.vivax antigen in which the plates were coated with rabbit anti-P.cynomolgi poly-antibody and two monoclonal antibodies were used together for reaction. The coincidence rate with microscopically conformed P.vivax blood samples was 94.3% and the coincidence rate with microscopically negative blood samples was 96.1%. The sensitivity was over 1 parasite/106 erythrocytes.

STUDY ON DETECTING BLOOD STAGE P.FALCIPARUM ANTIGEN BY USING SANDWICHED MONOCLONAL ANTIBODY-ELISA TEST / 中国血吸虫病防治杂志

0.05) The sensitvity is over 5 parasites/108 RBC. However, it is not satisfactory by using this method to detect P.vivax antigen.