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0.05). HL activity in liver tissue in AS group was significantly lower than control group (P
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AIM: To investigate the effect of tea-polyphe nols (TP) on the activation of NF-?B and the expression of TGF-? 1 mRNA in TH P -1 cells (a human acute monocytic leukemia cell line). METHODS: TH P-1 cells were incubated with the different concentrations of TP, VLDL, LDL or o x-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-?B was detected with immunohistochemistry technique, the positive index of the TGF-? 1 mRNA ex p ression was detected by hybridization in situ, and accumulation of total cholest erol (TC) in cells incubated with 0 4-40 ?g/L TP was determined with oxidase a ssay. RESULTS: The nuclear malposition rate of NF-?B, the posit ive index of the TGF-? 1 mRNA expression and TC in THP-1 cells incubated with 0 4-40 ?g/L of TP were lower than those with 0 ?g/L of TP in TP-V group, TP-L group and TP-O ( P 0.05). CONCLUSION: TP inhibited the activation of NF-?B, the expressio n of TGF-? 1 mRNA and the foam cell formation in the mono-macrophage.
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AIM: To investigate the effect of insulin on ox-LDL transferring the THP-1 cells to foam cells and influencing the LPL mRNA expression in THP-1 cells. METHODS: THP-1 cells were incubated with 50 mg/L ox-LDL and insulin at concentrations of 10 mU/L,100 mU/L,1 000 mU/L and 10 000 mU/L, respectively. The expression of LPL mRNA in cells was detected by RT-PCR. Lipoprotein lipase of THP-1 cells was presented by no-specific lipase staining. THP-1 cells were stained with oil red O. Accumulation of total cholesterol (TC) in THP-1 cells was determined with oxidase assay. RESULTS: In 100 mU/L?1 000 mU/L?10 000 mU/L insulin groups, LPL mRNA expression increased 2 times, the average cell perilength was longer, the percentage of positive oil red O staining cells was significant higher, the content of cholesterol in THP-1 cells was higher than in ox-LDL control ( P