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Artigo em Chinês | WPRIM | ID: wpr-263827

RESUMO

<p><b>OBJECTIVE</b>To study in the linkage between eotaxin-3 gene polymorphisms and allergic asthma susceptivity, blood plasma IgE or peripheral blood eosinophil in adult population of Han nationality from Hubei province of China.</p><p><b>METHODS</b>Polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), tetra-primer PCR technique and restriction analysis were applied to identify the single nucleotide polymorphism.</p><p><b>RESULTS</b>The allele frequency of eotaxin-3 +2497 G, total levels of plasma IgE and peripheral blood eosinophil counts revealed the significant difference between control and allergic asthma group, that the P value was 0.011, 0.021 or 0.029 respectively. The allele frequency of eotaxin-3 +77 T and total levels of plasma IgE showed to have no significant difference between control and allergic asthma group, that the P value was 0.824 and 0.473 respectively. However, the peripheral blood eosinophil counts was significantly different between control and allergic asthma group, and the P value was 0.044.</p><p><b>CONCLUSION</b>Single nucleotide polymorphism of eotaxin-3 +2497 is associated with the asthma susceptibility, peripheral eosinophil counts and total levels of plasma IgE in adult population from Hubei province, and polymorphism of +77 is associated with peripheral eosinophil counts.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Alelos , Asma , Genética , Alergia e Imunologia , Sequência de Bases , Quimiocina CCL26 , Quimiocinas CC , Genética , China , Etnologia , Análise Mutacional de DNA , Predisposição Genética para Doença , Imunoglobulina E , Sangue , Dados de Sequência Molecular , Polimorfismo Genético
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