Exploration of rational drug use administration at Beijing Chaoyang Hospital / 中华医院管理杂志

Beijing Chaoyang Hospital of Capital Medical University has been designated as a pilot hospital for introduction of contemporary hospital management system. It actively explored a new model of rational drug use administration, namely setting up chief pharmacists, pharmacists classified management, precision drug use service, and strengthened management of rational drug use. These efforts have efficiently minimized patients′ burden of drug expenditure, and enhanced incentives of the pharmacists, achieving the pharmacy′s functional transformation and value enhancement.

Exploration of rational drug use administration at Beijing Chaoyang Hospital / 中华医院管理杂志

Beijing Chaoyang Hospital of Capital Medical University has been designated as a pilot hospital for introduction of contemporary hospital management system. It actively explored a new model of rational drug use administration, namely setting up chief pharmacists, pharmacists classified management, precision drug use service, and strengthened management of rational drug use. These efforts have efficiently minimized patients′burden of drug expenditure, and enhanced incentives of the pharmacists, achieving the pharmacy′s functional transformation and value enhancement.

Rapid determination of fluoxetine in human plasma by LC-MS/MS / 国际检验医学杂志

Objective To establish LC‐MS/MS method for the determination of fluoxetine in human plasma .Methods After protein precipitation of acetonitrile the plasma sample was separated on an Agilent XDB‐C18 column using acetonitrile‐1 mmol/L ammonium formate(containing 0 .1% formic acid) as mobile phase by gradient elution .Detection was carried out by multiple reac‐tion monitoring(MRM) on 3200QTRAP LC‐MS/MS system .Results The assay was linear over the range 0 .30 -50 .0 ng/mL with a lower limit of quantitation of 0 .30 ng/mL .Intra‐and inter‐day precision were less than 15% ,respectively .The relative devia‐tion was in the range -2 .80% -2 .09% .The recovery of fluoxetine was more than 98% with less matrix effects .The stabilities were good .Conclusion It could be a rapid ,sensitive ,selective and reliable method for the determination of fluoxetine in human plas‐ma for therapeutic drug monitoring and pharmacokinetics .

Ultra High Performance Liquid Chromatography Coupled with Q Exactive Hybrid Quadrupole-orbitrap Mass Spectrometry for Serumal Metabonomics Study of Drug-induced Liver Injury in Patients / 分析化学

Ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometry ( UPLC-MS/MS) and multivariate statistical analysis was applied to metabonomic study of drug-induced liver injury. This investigation focused on the liver injury in clinic which was induced by antitumor drug, traditional Chinese medicine, statins and antibiotics. After precipitated by cold acetonitrile, the serum samples were separated on a HSS T3-C18 column using water (containning 0. 01% formic acid) and acetonitrile as mobile phase by gradient elution. Based on metabonomic profiles analysis of the orthogonal projections to latent structures discriminant analysis ( OPLS-DA ) and partial least squares-discriminant analysis ( PLS-DA) , 114 endogenous metabolites had been found to have significant differences between drug-induced liver injury patients and healthy volunteers, 38 endogenous metabolites have significant difference (p<0. 001). According to the results of the OPLS-DA, MS/MS data and human metabolome database ( HMDB) , phenylalanine and dimethylguanosine had been identified as potential biomarkers related to drug-induced liver injury for early discovery and diagnosis.

Dimethyl sulfoxide acts as a protective agent to perfuse rabbit amputated limbs:the relative recovery of local drug concentrations / 中国组织工程研究

BACKGROUND:The effect of dimethyl sulfoxide cryoprotectants has been got a lot of verification in the low-temperature medical applications. But there is no literature addressing microdialysis detection of dimethyl sulfoxide cryoprotectants. OBJECTIVE:To investigate the microdialysis relative recovery of different concentrations of dimethyl sulfoxide cryoprotectants used for limb reattachment. METHODS:In vitro linear probe relative recovery of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) was detected by retrodialysis gain and loss method. The correction in vivo experiment was done to estimate dimethyl sulfoxide relative recovery in rabbit amputated limbs. RESULTS AND CONCLUSION:The relative recoveries of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) were (49.49±3.56)%, (46.30±1.48)%, (52.66±2.54)%using retrodialysis gain method and (50.99±6.89)%, (43.86±1.35)%, (50.67±0.75)%using retrodialysis loss method. The average recoveries were (49.48±3.18)%and (48.51±4.03)%, respectively. There was no difference in the relative recovery of dimethyl sulfoxide detected using two methods. The change of dimethyl sulfoxide concentration could not influence the retrodialysis gain and loss method calibration results. The recovery was (15.45±4.8)%in vivo. These findings indicate that the microdialysis technology is suitable for dimethyl sulfoxide sampling in vivo that has no obvious influence on the relative recovery.