A family-based transmission disequilibrium test of polymorphisms of endothelial nitric oxide synthase gene in children with orthostatic intolerance / 中国当代儿科杂志

<p><b>OBJECTIVE</b>Recent studies have found that the variation of G894T on the region of T786C and 7th exon promoted by endothelial nitric oxide synthase (eNOS) gene is associated with cardiovascular disease. This research explored possible correlations between eNOS gene polymorphisms and orthostatic intolerance (OI) in children through linkage disequilibrium analysis between eNOS genes T786C and G894T and OI.</p><p><b>METHODS</b>PCR, Macrorestriction Map and other molecular biotechnology were used to determine the genotypes of eNOS/T786C and G894T in 60 OI probands and their parents. Correlation analysis and transmission disequilibrium test (TDT) between T786C, G894T and OI were performed.</p><p><b>RESULTS</b>There was linkage disequilibrium of eNOS/T786C and G894T gene polymorphisms in the occurrence of childhood OI (P<0.05).</p><p><b>CONCLUSIONS</b>eNOS genes T786C and G894T may be associated with the pathogenesis of OI.</p>

Xianling Gubao Capsule improves semen quality / 中华男科学杂志

<p><b>OBJECTIVE</b>To observe the effect of the compound Chinese traditional drug Xianling Gubao Capsule on the semen quality of infertile males.</p><p><b>METHODS</b>We treated 66 infertile men with Xianling Gubao Capsule for 24 months, and analyzed the semen quality and sperm morphology before and after the treatment.</p><p><b>RESULTS</b>Two months after the medication, sperm concentration was increased by a small margin, but no statistically significant changes were observed in sperm vitality and motility (P > 0.05), the rate of morphologically normal sperm was significantly raised from 25.8% before treatment to 57.6% (P < 0.05) in those with the normal rate > or = 15%, but decreased from 53.0% to 25.8% (P < 0.05) in those with the normal rate < 9%. Among the 7 cases of oligospermia, the rate of morphologically normal sperm was elevated to an average of 10.9% after the 4-month medication, significantly different from the baseline rate of 5.8% (P < 0.05). Five spontaneous pregnancies and 1 successful IVF-ICSI were achieved during the treatment.</p><p><b>CONCLUSION</b>Xianling Gubao Capsule can improve semen quality and significantly increase the percentage of morphologically normal sperm.</p>