Selection and application of biodegradable scaffold in tracheal tissue engineering / 国际生物医学工程杂志

Long tracheal lesions are mainly caused by stenosis, infection, trauma, malignant tumors and other factors. Resection of the diseased tissue or stenosis and end-to-end anastomosis is currently the gold standard for long tracheal lesions treatment. However, these treatment programs have proven to have major limitations. In recent years, tissue engineering technology has been regarded as a promising medical alternative treatment method, and the selection of scaffold materials is one of key parts. With the continuous exploration of domestic and foreign researchers, biological materials have been continuously developed and applied to the research of tissue engineering trachea. Tissue engineering degradable scaffold materials can be divided into natural polymer material scaffolds and synthetic polymer scaffolds according to the different sources. The scaffold material can be modified or compounded as needed to improve the biological properties of scaffolds. In addition, with the continuous development of biological printing technology, different scaffold materials can be better combined and used. Biodegradable scaffolds have become a new research direction in the field of tissue engineering trachea due to their polymer properties, and have good application prospects.

Effect of genipin cross-linking on the biocompatibility of rabbit acellular tracheal matrix / 国际生物医学工程杂志

Objective:To investigate the biocompatible properties of tissue-engineered rabbit trachea treated by Triton-X 100 processed method (TPM) and detergent enzymatic method (DEM) with genipin cross-linking.Methods:TPM and DEM were used to decellularize New Zealand rabbit trachea, and then genipin was used for cross-linking. The mechanical properties of each tracheal sample were measured by universal tensile testing machine. The structure of the sample was observed by scanning electron microscope. The cytotoxicity of the sample was detected by cell contact toxicity assay. Fifteen healthy adult New Zealand rabbits with no specific pathogens were divided into the native tracheal transplantation group, the genipin cross-linked TPM acellular tracheal matrix transplantation group and the genipin cross-linked DEM acellular tracheal matrix transplantation group according to the random number table, 5 animals for each group. Animals in each group were sacrificed 30 days after transplantation, and graft samples were obtained. The microstructure was observed by hematoxylin-eosin staining and CD68 molecular immunohistochemical staining.Results:Biomechanical results showed that the mechanical properties of decellularized tracheas with genipin cross-linking were similar to native tracheas. The results of scanning electron microscopy showed that the matrix of cross-linked decellularized tracheas was more dense comparing with native tracheas, and the mesh-like ultrastructure formed on the outer surface of the genipin cross-linked DEM acellular tracheal matrix was conducive to cell adhesion. The results of cell contact toxicity results showed that the genipin cross-linked decellularized tracheas treated by DEM had better biocompatibility. The results of in vivo implantation and histological staining showed that genipin cross-linked DEM acellular tracheal matrix was less immunogenic comparing with genipin cross-linked TPM acellular tracheal matrix.Conclusions:Genipin can improve the ultrastructure of decellularized tracheal matrix without causing inflammatory. The genipin cross-linked decellularized tracheas treated by DEM has better biocompatibility and lower immunogenicity, which make it suitable for the replacement of tissue engineering trachea.