RESUMO
This study describes the use of an IgM capture ELISA using cell culture derived antigens and a polyclonal rabbit antiflavivirus antisera for the detection of dengue positive cases. The IgM capture ELISA is compared with the dot enzyme immunoassay and the results are discussed in the context of dengue endemicity.
Assuntos
Dengue/sangue , Ensaio de Imunoadsorção Enzimática/normas , Estudos de Avaliação como Assunto , Humanos , Immunoblotting/normas , Técnicas Imunoenzimáticas/normas , Imunoglobulina M/sangue , Malásia/epidemiologia , Recidiva , Sensibilidade e Especificidade , SorotipagemRESUMO
This study describes the status of viral encephalitis in Perak, Malaysia during the year 1990. In addition, 14 cases selected from Penang and Perak during the years 1989 and 1990 are presented, with data showing titers of neutralizing antibodies against Japanese encephalitis virus (JEV) and dengue 2 virus, titers of antibodies against JEV and dengue virus antigens as determined by DEIA, and a comparison of these with the presence of IgM to JEV and dengue virus. These data show that there probably is far more viral encephalitis due to JEV in Malaysia than the national figures reflect.