Estudo da ação citotóxica de drogas antineoplásicas associadas ou não à dexametasona sobre as células de mastocitoma murino (linhagem p-815)

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Mononuclear cell cytotoxicity in rats with Walker 256 tumor

In the present study we characterized the cytotoxicity of Wistar rat mononuclear cells from 21 animals which received 10(6) Walker 256 tumor cells by the subcutaneous route. All animals developed the tumor. Cytotoxicity was studied 15 days after inoculation using spleen, thymus and lymph node T lymphocytes as well as macrophages from the peritoneal cavity. A Walker 256 tumor cell suspension and tumor cells in culture (YAC-1) were labelled with 51Cr and used as target cells, according to the Herberman technique and a gamma counter was used for counting. Anti-Walker cell cytotoxicity was significantly decreased in T lymphocytes from the spleen (9.6 vs 51.1 for the control) and thymus (11.5 vs 38.2 for the control), whereas no difference was observed for lymph nodes (41.2 vs 49.5 for the control) or macrophages (43.4 vs 46.3 for the control). Anti-YAC-1 cytotoxicity was significantly decreased in T lymphocytes from all lymphoid organs compared to control: 23.6 vs 42.8 for the spleen, 22.6 vs 41.1 for the thymus, 26.6 vs 42.1 for lymph nodes, and 27.1 vs 46.3 for macrophages. No correlation was observed between tumor weight, and anti-Walker cytotoxicity or anti-YAC-1 cytotoxicity.

Antitumor effects of rhodium (II) complexes on mice bearing Ehrlich tumors

Rhodium (II) trifluoracetate (TFARh), rhodium (II) trifluoracetate adduct with sulfadiazine (TFARh.Sd) and rhodium (II) acetate adduct with sulfisoxazole (RhSx) were tested in mice for acute toxicity, antitumoral activity against Ehrlich ascites carcinoma and for viability of Ehrlich tumor cells in culture. At ip doses up to 60 µmg/kg (40-70 and 59 mg/kg, respectively), these coumpounds had no toxic effects up to 14 days. At ip doses of 10 µmol Kg-1 day-1 for 5 days, TFARh and TFARh.Sd significantly increased the survival rate of mice bearing Ehrlich ascites cells (probability of survival to the end of 34th day, controls = 0.23, TFARh = 0.85, TFARh.Sd = 0.74). No significant effect was observed for RhSx. In vitro, these rhodium complexes at 40 µM significantly increased the number of dead cells in cultured Ehrlich tumor cells

Antitumor effects of Rhodium (II) citrate in mice bearing Chrlich tumors

Rhodium II citrate was tested in mice for acute toxicity, antitumoral activity against Ehrlich ascites carcinoma and inhibition of DNA synthesis by Ehrlich tumor, malignant adrenocortical cells (Y-1) and normal adrenocortical cells (AR-1)_. At ip doses up to 260 mg/Kg, the compound had no toxic effects for up to 14 days. The same total dose given over 4 days significantly increased the survial rat of mice bearing Ehrlich ascites cells. Thymidine incorporation by Ehrlkich tumor, Y-1 cells in vitro was inhibited 50% by a.1 to 0.2 mM concentrations of the compound. We conclude that the increase survival of the tumor-bearing mice was due at least in part to the inhibition of DNA synthesis with a consequet reduction of cell division and tumor growth

Aspectos basicos das linhagens celulares mais utilizadas. / Culture characteristics of some cell lines

Sao apresentados alguns aspectos basicos das linhagens celulares mais utilizadas nos nossos laboratorios de pesquisas. Quatro linhagens de origem humana e tres linhagens de origem animal sao descritas quanto a sua obtencao, meio de congelamento, meio de cultura, caracteristicas de crescimento e morfologia

Naftil-azoderivado da sulfamerazina: - toxicidade aguda e alguns efeitos colaterais no sitema nervoso central. / Sulfamerazine naftilazoderivate: - Acute toxicity and some adverse effects on central nervous system

Neste trabalho visou-se estudar comparativamente as acoes comportamentais do naftil-azoderivado da sulfamerazina e da propria sulfamerazina. Observou-se que o naftil-azoderivado apresenta atuacao que comprometem o comportamento aprendido diminuindo as respostas em ratos condicionados. A movimentacao espontanea, observada em campo aberto, em camundongos sob acao do naftil-azoderivado, aumentou com o decorrer do tempo, tendo atingindo o seu maximo apos 24 horas da injecao intraperitoneal. A toxicidade aguda do naftil-azoderivado diminuiu em relacao a sulfamerazina, quando da determinacao da DL 50. O composto ja avaliado anteriormente continuou a apresentar atuacao antimicrobiana