RESUMO
Objective To study the effect of Shuangteng Bitong Cataplasm on TLR4, MyD88 and NF-κB and inflammatory cytokines in rats with collagen-induced arthritis; To discuss relevant mechanism of action. Methods A CIA model was made by subcutaneous injection of collagen emulsion from rat tail. Rats were randomly divided into normal group,model group,Shuangteng Bitong Cataplasm high-dose and low-dose groups.The contents of TNF-α, IL-1β and IL-6 in plasma were evaluated by ELISA, and the expression levels of TLR4, MyD88 and NF-κB in synovial tissues were evaluated by RT-PCR and Western blot. Results Compared with the model group, the contents of TNF-α, IL-1β and IL-6 in serum decreased remarkably in the Shuangteng Bitong Cataplasm low-dose and high-dose group, and the expression levels of TLR4, MyD88 and NF-κB in synovial tissues decreased remarkably. Conclusion Shuangteng Bitong Cataplasm can inhibit inflammatory reaction and regulate the expressions of relevant factors in synovial tissues to realize the anti-flammatory effects.
RESUMO
Objective: To study the allelopathic effect of water extract from Tridax procumbens (WETP) on the seed germination and seedling growth of four kinds of crops. Methods: By water extraction, the stems, leaves, and seeds of T. procumbens were extracted, respectively. The seeds and seedlings of the four kinds of crops were treated with WETP, using distilled water as a control, and the differences between the seeds at four kinds of concentration were analyzed. Results: The synthetic action of WETP on Vigna radiata is promoting. With the increasing concentration, the promoting is diminished. But the effects of WETP on Raphanus sativus, Brassica campestris, and Lactuca sativa were promoting at low concentration and inhibition at high concentration. The inhibitory sequences of water extracts of different parts were seeds > leaves > stems, and for the seeds, L. sativa > R. sativus > B. campestris > V. radiata. The inhibition on L. sativa was the most significant. Conclusion: T. procumbens has the allelopathic effect on native plants. Therefore, T. procumbens could be used as herbicide and cover plant for competition to inhibit other weeds. It has a development and application value.