RESUMO
Aim To investigate the effect of astragalo- side Ⅳ on focal cerebral ischemia-reperfusion injury in rats.Methods The focal cerebral ischemia/reper-fusion of rat left middle cerebral artery occlusion ( MCAO) was induced by suture method .Male SD rats were randomly divided into sham operation group , cer-ebral ischemia/reperfusion group , astragaloside IV group and solvent control group .Except for the sham operation group , the others were subjected to ischemia 2h and reperfusion 24h.Then, rats with successful model were chosen for the detection of various indexes . Astragaloside IV group was injected intraperitoneally with astragaloside IV(20 mg· kg -1 ) at the same time as reperfusion , while solvent control group was injected with the same amount of solvent .TTC staining was used to detect the volume of cerebral infarction , and Nissl staining to observe the changes of histomorpholo-gy, and transmission electron microscope (TEM) to ob-serve the ultrastructure of the cells .Results There was no neurological deficit in the sham operation group, and the volume of cerebral infarction was zero . Compared with the sham operation group , there were some increased neurological deficits , nerve cell damage and cerebral infarction volume in other groups ( P <0.05) .Compared with the cerebral ischemia/reperfu-sion group , the nerve function damage could be signifi-cantly improved , the damage of neurons reduced , and the volume of cerebral infarction decreased ( P<0.05 ) in astragaloside IV group , and there was no obvious change in the solvent control group ( P>0.05 ) .Con-clusion Astragaloside IV can reduce the focal ische-mia/reperfusion injury in rats and protect nerve cells from damage.