RESUMO
The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 (CHRM1) expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 (GLI1), patched 1 (PTCH1), and sonic hedgehog (SHH) expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hedgehog signaling pathway.
Assuntos
Humanos , Masculino , Carbacol/farmacologia , Movimento Celular/genética , Proliferação de Células , Proteínas Hedgehog/genética , Agonistas Muscarínicos/farmacologia , Antagonistas Muscarínicos/farmacologia , Receptor Patched-1/genética , Pirenzepina/farmacologia , Neoplasias da Próstata/patologia , Receptor Muscarínico M1/genética , Proteína GLI1 em Dedos de Zinco/genéticaRESUMO
Objective To analyze the incidence trend of malignant tumor in Minhang District of Shanghai from 2002 to 2013,in order to provide scientific evidence for making the comprehensive prevention and control strategy.Methods Incidence of cancer data (2002-2013) of Minhang District were collected.The crude rate,standardized rate,sectional shrinkage rate,accumulation rate,percentage change (PC),annual percentage change (APC) and annual change contribution rate were calculated for main malignant tumors separately.Results The total incidence of malignant tumors in Minhang District from 2002 to 2013 increased year by year,mainly showed in thyroid cancer,colorectal cancer and breast cancer,while decreased in gastric cancer,liver cancer and lung cancer.Conclusions The incidence of malignant tumor in Minhang District was increasing.The incidence of thyroid cancer,colorectal cancer and breast cancer were significantly increased,which were needed to pay more attention.The incidence of gastric cancer and liver cancer were decreased significantly,which indicated that exposure factors were controlled effectively.
RESUMO
Objective To investigate the risk of overall and site-specific cancers in Chinese patients with type 2 diabetes mellitus (T2DM) in Minhang District of Shanghai.Methods A population-based retrospective cohort study including 51 372 T2DM patients was conducted in Minhang District,Shanghai from 2004 to 2014.All T2DM patients were enrolled from the standardized management system based on local electronic information system of Shanghai.Newly-diagnosed cancer cases were identified by record-linkage with the Shanghai Cancer Registry.Standardized incidence ratios (SIR) and 95％CI were used to estimate the risk of cancers among T2DM patients.Results Overall crude incidence rate (CIR) of cancers was 969.69 per 105 person-years in men and 834.17 per 105 person-years in women.The age-standardized rate (ASR [W]) was 206.72 per 105 person-years in men and 285.80 per 105 person-years in women.Increased risk of cancer was found in both sex,with an SIR of 0.93 (95％CI:0.88-0.98) in men and 1.07 (95％ CI:1.01-1.12) in women.Conclusions Our results indicate that patients with T2DM may have an increased risk of some cancers,and the increase may vary by sub-sites of cancers.
RESUMO
Objective To analyze the incidence trend of malignant tumor in Minhang District of Shanghai from 2002 to 2013,in order to provide scientific evidence for making the comprehensive prevention and control strategy.Methods Incidence of cancer data (2002-2013) of Minhang District were collected.The crude rate,standardized rate,sectional shrinkage rate,accumulation rate,percentage change (PC),annual percentage change (APC) and annual change contribution rate were calculated for main malignant tumors separately.Results The total incidence of malignant tumors in Minhang District from 2002 to 2013 increased year by year,mainly showed in thyroid cancer,colorectal cancer and breast cancer,while decreased in gastric cancer,liver cancer and lung cancer.Conclusions The incidence of malignant tumor in Minhang District was increasing.The incidence of thyroid cancer,colorectal cancer and breast cancer were significantly increased,which were needed to pay more attention.The incidence of gastric cancer and liver cancer were decreased significantly,which indicated that exposure factors were controlled effectively.
RESUMO
Objective To investigate the risk of overall and site-specific cancers in Chinese patients with type 2 diabetes mellitus (T2DM) in Minhang District of Shanghai.Methods A population-based retrospective cohort study including 51 372 T2DM patients was conducted in Minhang District,Shanghai from 2004 to 2014.All T2DM patients were enrolled from the standardized management system based on local electronic information system of Shanghai.Newly-diagnosed cancer cases were identified by record-linkage with the Shanghai Cancer Registry.Standardized incidence ratios (SIR) and 95％CI were used to estimate the risk of cancers among T2DM patients.Results Overall crude incidence rate (CIR) of cancers was 969.69 per 105 person-years in men and 834.17 per 105 person-years in women.The age-standardized rate (ASR [W]) was 206.72 per 105 person-years in men and 285.80 per 105 person-years in women.Increased risk of cancer was found in both sex,with an SIR of 0.93 (95％CI:0.88-0.98) in men and 1.07 (95％ CI:1.01-1.12) in women.Conclusions Our results indicate that patients with T2DM may have an increased risk of some cancers,and the increase may vary by sub-sites of cancers.
RESUMO
<p><b>OBJECTIVE</b>To evaluate the diagnostic value of full-field digital mammography for breast cancer.</p><p><b>METHODS</b>The clinical data and mammograms of 230 patients with breast diseases between January 2008 and July 2008 were collected and reviewed. Craniocaudal (CC) and mediolateral oblique (MLO) view mammograms were performed in all patients before surgery. Three experienced radiologists in breast imaging assessment analyzed and classified all the mammograms according to breast imaging reporting and data system (BI-RADS). The sensitivity, specificity and accuracy were evaluated according to their pathological diagnosis. The reasons resulting in false-negative and false-positive diagnosis were also analyzed.</p><p><b>RESULTS</b>Of the 238 samples, 130 had a malignant breast tumors and 108 cases of benign breast lesions. One hundred and nine of the 130 malignant breast tumors were invasive ductal carcinoma. Fifty-seven of the 108 benign breast lesions were breast adenosis. Masses or masses with microcalcification were the most frequently seen signs of the malignant tumors, accounting for 40.8% and 20.8%, respectively. The sensitivity, specificity and accuracy of FFDM in detecting breast carcinoma were 90.8%, 87.0% and 89.1%, respectively. The false-negative signs including negative X-ray finding (5 cases) and focal asymmetric densities (4 cases). The false-positive signs were masses with spiculate, indistinctive or lobulated margin leading to misdiagnosing the lesions as malignant tumors.</p><p><b>CONCLUSION</b>Full-field digital mammography (FFDM) is helpful in detection of breast cancers in women, with a higher sensitivity, specificity and accuracy, and has an important clinical application value.</p>