RESUMO
<p><b>OBJECTIVE</b>To investigate the inhibitory effects of recombinant human endostatin (rh-ES) on cell adhesion, metastatic potential and invasiveness of hepatocellular carcinoma HCCLM6 cells in vitro.</p><p><b>METHODS</b>The changes in the cell proliferation status of HCCLM6 cells treated with different concentrations of rh-Endostatin in vitro was measured with MTT assay, and their invasiveness and migration were assayed using transwell cell culture chamber. The cell adhesion assay was carried out on 96-well plate precoated with matrigel.</p><p><b>RESULTS</b>Rh-ES showed inhibitory effect against the proliferation of HCCLM6 cells after a 72-h treatment. The adhesion, metastatic potential and invasiveness of the cells were obviously inhibited by rh-Endostatin in a dose-dependent manner.</p><p><b>CONCLUSION</b>Rh-ES inhibits the adhesion, invasiveness and migration of hepatocellular carcinoma cells in vitro, and the mechanism needs further investigation.</p>
Assuntos
Humanos , Antineoplásicos , Farmacologia , Carcinoma Hepatocelular , Patologia , Adesão Celular , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Sobrevivência Celular , Relação Dose-Resposta a Droga , Endostatinas , Genética , Farmacologia , Neoplasias Hepáticas , Patologia , Invasividade Neoplásica , Metástase Neoplásica , Proteínas Recombinantes , FarmacologiaRESUMO
Objective To explore the feature of clinical and laboratory and therapy in childern with idiopathic pulmonary hemosiderlsis(IPH).Methods The clinical and laboratory datas of 50 cases with IPH were studied from Jan.1996 to Jan.2006.The hemogram,the metabolism of ferrum,the cellular test of bone marrow,finding the hemosiderin cells in sputum or gastric juice or bronchovesicular juice and ma-nifestation were analyzed.Results IPH were often found in children aged 6 to 14 years old.Coungh and spitting blood and anemia were the most important clinical feature.X-ray manifestations were various.The diagnosis of IPH was based on clinical feature and finding out the he-mosiderin cells in sputum or gastric juice or bronchovesicular juice.X-ray and CT manifestations were important.At the same time,the secon-dary IPH was removed.The clinical symptom of IPH were relieved by using adrenal cortical hormone.Conclusions There is no specificity in children with IPH.X-ray,CT manifestations and finding the hemosiderin cells in sputum or gastric juice are helpful in diagnosis.Using adrenal cortical hormone will release symptom and prognosis.