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Objective To analyze the correlation between pathological of non small cell lung cancer(NSCLC)and mutations of the epidermal growth factor receptor(EGFR)gene.Methods Selected 96 patients with lung cancer for EGFR mutation de-tection in Shaanxi Provincial People's Hospital from January 2014 and April 2015,comparison of different types of NSCLC EGFR mutation rate.Results Gene mutation sensitive in 24 cases,in 12 cases and location of the mutation L858R,10 cases of mutation at position 19DEL,1 cases of mutation position G719X and S768I,1 cases EML4-ALK fusion.Mutation rate of 29.17%.Slightly lower than the international and domestic NSCLC epidermal growth factor receptor gene mutation sensitive rate of 30% of the average.Conclusion Target to treatment is aimed at the effective means for the treatment of NSCLC pa-tients with individualized,has reliable efficacy,less toxicity and adverse reactions,will become one of the most promising treatment,the study results below average,less attention to the base of the study population and need to raise awareness of routine testing.
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[Objective]To investigate the effect of human umbilical cord mesenchymal stem cells on infected state of human alve?olar type Ⅱ epithelial cells.[Methods]Human alveolar type Ⅱ epithelial cells A549(1×105/mL)2 mL and PA(3×104 CFU/mL)2 mL has grown after 6 hours,add hUCMSC(1 × 106/mL)2 mL as the experimental group,add equal amounts of phosphate buffer (PBS)for infection,A549 and PBS and the medium has grown as the control group. A549 cells morphological changes between the compared groups(Transmission electron microscope,TEM),A549 cell viability(new CCK-8 cell proliferation assay Kit),A549 cells apoptosis(Annexin V-FITC/PI double staining flow cytometry)and the expression of A549 pulmonary surfactant A(SP-A) (Western blot).[Results]Transmission electron microscope cell morphology observation displayed ,infection group A549 cell dam?aged obviously,cell quality appeared empty bubble degeneration,chromatin height agglutination,visible apoptosis bodies;experi?ment group cell package film structure full,nuclear film full,nucleolus obviously,nuclear chromatin electronic density low,chroma? tin uniform,no apoptotic bodies;control group A549 cell structure full,membrane surface micro-fluff rich,nuclear film full,nucle?ar week clearance structure normal,chromatin uniform;infection group and control group compared,Infection group A549 cell sur?vival significantly reduced[(70.35±2.89)% and(97.37±2.07)%,n=3,P<0.01],apoptosis rate significantly increased[(8.63%± 0.16)%and(2.55±0.11)%,n=3,P<0.01],In the infected group,PA could damage A549 cells and decrease the amount of SP-A ex?pression(n=5,P<0.05). In the experiment group,the protective effect of hUCMSC on the A549 cells after infection may increase the expression of SP-A(n=5,P<0.05);[Conclusions]HUCMSC inhibits the infection of A549 cells apoptosis and protection of A549 cells secrete SP-A.