Real-time PCR quantification of Treponema denticola in subgingival plaque from patients with different severities of chronic periodontitis / 中国组织工程研究

BACKGROUND:Oral treponemes (especially Treponema denticola) are widely considered to play important roles in the occurrence and development of chronic peridontitis.Treponema denticola can be detected in both periodontal patients and healthy individuals,so its pathogenicity may depend on its relative numbers in subgingival plaque.OBJECTIVE:To detect the number of Treponema denticola in subgingival plaque of patients with different severities of chronic periodontitis and to investigate the relationship between the bacterial colonization and the periodontal status.METHODS:Subgingival plaque samples were respectively taken from 132 patients with chronic periodontitis and 40 healthy controls,admitted in the Department of Stomatology,Aviation General Hospital of Chinese Medical University from July 2015 to August 2016.Based on the clinical data,132 patients were divided into mild (n=41),moderate (n=46),and severe (n=45) groups.The presence,relative numbers and proportion of Treponema denticola in the samples were detected by TaqMan real-time PCR assay.RESULTS AND CONCLUSION:There was no difference in the presence of Treponema dentico/a in subgingival plaque between healthy and periodontal participants.The relative numbers and proportion of Treponema denticola in the periodontal patients were significantly higher than those in the healthy controls (P ＜ 0.000 1).The quantification of Treponema denticola in different stages of periodontitis did show significant differences (P ＜ 0.05).In addition,the bacterial level and proportion of Treponema denticola were positively correlated to the probing depths (P ＜ 0.000 1).When the probing depth was more than 7 mm,the bacterial level and proportion of Treponema denticola were significantly increased (P ＜ 0.05,P ＜ 0.01).These findings suggest that Treponema denticola widely exists in subgingival plaque of human.The bacterial load and proportion of Treponema denticola are tightly related to the severity of periodontitis and probing depth.The higher bacterial level and proportion of Treponema denticola indicate a severer periodontitis.Moreover,real-time PCR possesses a broad potential in the studies on etiology,diagnosis and treatment of pedodontology.

Establishment and application of management system of medical adverse events / 医疗卫生装备

Objective To establish a medical adverse events management system to enhance medical quality. Methods An adverse events management module was embedded into the existing electronic medical record quality control program. Structural options and short text on medical adverse events constituted the medical adverse events report, which posed emphases on the cases determined by keyword screening or with high-risk conditions. The report contributed to early warning against suspected medical adverse events, and thus the report rate could be increased. Results The trials showed that the reported cases were increased by 139.68%when compared with that in last year, and enhancement also occurred in medical staff enthusiasm as well as the efficiency and accuracy of the report. Conclusion The system behaves well in medical links supervision, medical risk warning, medical service enhancement and patient safety improvement, and thus is worthy promoting practically.