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1.
Chinese Journal of Clinical Laboratory Science ; (12): 644-648, 2017.
Artigo em Chinês | WPRIM | ID: wpr-666702

RESUMO

Objective To develop a high-performance liquid chromatography (HPLC) method for the measurement of lecithin-cholesterol acyltransferase(LCAT) activity and analyze the relationships between LCAT activity and the traditional risk factors of atherosclerotic cardiovascular disease(CVD).Methods The liposome which contained 7-dehydrocholesterol and 1,2-didecanoyl-sn-glycero-3-phosphocholine (10∶0 PC)as the substrate of LCAT and LCAT activating peptide (LAP642)as LCAT activator was mixed with 10 microliters of serum sample(50∶ 1,V/V)in ice-water bath and subsequently incubated at 37 ℃ for 1 h.After extracting with hexane,the lipid was analyzed by HPLC and the LCAT activity was calculated as the ratio of 7-dehydrocholesterol ester to free 7-dehydroeholesterol.LCAT activities of 120 health volunteers were measured and its relationship with traditional risk factors of CVD was analyzed.Results The liposome composed of substrates(7-dehydrocholesterol and 10∶0 PC with ratio of amount 1∶ 8.5)and LAP642 was stable,efficient and easy for preparation.LCAT activity was a linear correction during 8 hours of incubation and was independent of the volume of serum added in the range from 0 to 20 microliters.The averages of intra-and total coefficients of variation(CV)were less than 1.76% and 3.11% respectively.The comparison of two methods showed that the results of the HPLC method were highly correlated with LCAT mass measured by commercial ELISA method and LCAT activity measured by endogenous substrate fractional esterification of high density lipoprotein cholesterol (FERHDL)(P < 0.01).LCAT activity positively correlated with body mass index(BMI),triglyceride (TG) (P < 0.05) and negatively correlated with apolipoprotein AI (apoAI) (P < 0.05) and high density lipoprotein cholesterol (HDL-C) (P < 0.01) in the volunteers.Conclusion A simple,precise and reliable HPLC method for determination of LCAT activity using artificial substrate has been established,and the results were not influenced by endogenous cholesterol levels in serum.The newly developed method could be a useful tool in the study of lipid metabolism and the assessment for risk factors of CVD.

2.
China Medical Equipment ; (12): 82-85, 2017.
Artigo em Chinês | WPRIM | ID: wpr-664318

RESUMO

Objective:To explore the impact and curative effect of radiofrequency ablation (RFA), laparoscopic surgery and total hysterectomy for ovarian function in the treatment of uterine fibroids.Methods: 60 patients with uterine fibroids were divided into RFA group (20 cases) and laparoscope group (20 cases) and panhysterectomy group (20 cases) as different therapeutic method. The serum follicle stimulating hormone (FSH) and estradiol (E2) of three groups in preoperative, postoperative 3th month and postoperative 6th month were detected, respectively. The influence and curative effect of different therapeutic method on ovarian function were compared and analyzed. Results: All of operations of three groups were successful at the first time, and the total effective rate of the three groups were 98% (19/20), 98% (19/20), 100% (20/20) at postoperative 3th months, respectively. And the total effective rate of the three groups were 90% (18/20), 98% (19/20), 100% (20/20) at postoperative 6th months, respectively. There was no significant difference between the 3th month and 6th month (F=5.350,F=8.319,P>0.05). The hormonal level of patients in three groups were abnormal at preoperative period, while at postoperative 3th month, the normal hormonal levels were, respectively, 60% (12/20) in RFA group, 50%(10/20) in laparoscope group. And the differences of each group between pre and post operation were significantly (t=7.938,t=8.362,P<0.05), respectively. And at the postoperative 3th month, the serum FSH and E2 of pan-hysterectomy group were no, respectively, significant higher and lower than that of preoperative results (t=2.017,P>0.05). At the postoperative 6th month, the normal hormonal level were, respectively, 70% (14/20) in RFA group, 65% (13/20) in laparoscope group. And the FSH and E2 of these groups were significantly higher and lower than that of pre-operation (t=10.918, t=11.218,P<0.05).Conclusion: All of the three methods has significant curative effect in the treatment of uterine fibroids, and the ovarian function is improved by using RFA and laparoscope while it is recession by using pan-hysterectomy.

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