Clinical analysis of 6 cases of acute neonatal suppurative appendicitis with perforation / 中国新生儿科杂志

Objective:To study the clinical features and risk factors of prognosis of neonatal appendicitis.Methods:From January 2014 to December 2022, all infants with neonatal appendicitis and received surgery in our hospital were retrospectively analyzed.Results:A total of 6 cases were enrolled, including 1 boy and 5 girls, with gestational age 36-40 weeks, birth weight 1 990~3 300 g, age of admission 5-11 d and time from illness onset to admission 0.5-4 d. All infants had abdominal distension, combined with vomiting in 4 cases, fever in 3 cases and blood in stool in 1 case. Gastrointestinal perforation was found on preoperative abdominal X-ray in 5 cases. All 6 cases received surgery and confirmed the diagnosis of appendicitis with perforation during the surgery. Appendectomy was performed without mortality. 1 case had Amyand hernia and received high ligation of the hernia sac during operation. 1 case had meningitis and was cured after 3 weeks of antibiotic treatment. 1 case developed adhesive intestinal obstruction 3 months after surgery and underwent intestinal adhesiolysis. One case developed colonic stenosis one month after surgery. The stenotic segment of the colon was resected and primary intestinal anastomosis was performed.Conclusions:Neonatal appendicitis progresses rapidly and is difficult to diagnose. The possibility of appendicitis with perforation should be considered when preoperative abdominal X-ray suggesting pneumoperitoneum. Intraoperatively, it is necessary to pay attention to the relationship between appendiceal perforation and other lesions for comprehensive treatment, and change the surgical approach accordingly.

TLR8 in the Trigeminal Ganglion Contributes to the Maintenance of Trigeminal Neuropathic Pain in Mice / 神经科学通报·英文版

Trigeminal neuropathic pain (TNP) is a significant health problem but the involved mechanism has not been completely elucidated. Toll-like receptors (TLRs) have recently been demonstrated to be expressed in the dorsal root ganglion and involved in chronic pain. Here, we show that TLR8 was persistently increased in the trigeminal ganglion (TG) neurons in model of TNP induced by partial infraorbital nerve ligation (pIONL). In addition, deletion or knockdown of Tlr8 in the TG attenuated pIONL-induced mechanical allodynia, reduced the activation of ERK and p38-MAPK, and decreased the expression of pro-inflammatory cytokines in the TG. Furthermore, intra-TG injection of the TLR8 agonist VTX-2337 induced pain hypersensitivity. VTX-2337 also increased the intracellular Ca

Circulating tumor cells in the diagnosis and treatment of early and advanced prostate cancer / 中华男科学杂志

Circulating tumor cells (CTC) are tumor cells that escape from the primary or metastatic tumor into the circulatory system, and closely related to cancer metastasis. Since the samples can be obtained through simple and minimally invasive blood sampling operations, CTCs have a great clinical potential. PCa is one of the most common malignant tumors in men. In recent years, many scholars have conducted studies as to whether CTC technology can be used for the diagnosis and treatment of PCa, as well as for more accurate prediction of the risk of progression. This article summarizes the advances in researches relating CTC technology and the diagnosis and treatment of PCa. CTC detection has been developed from simple counting to phenotypic classification, and even to its combination with the determination of the expressions of specific genes (such as AR, AR-V7, etc.) and single-cell sequencing. Some reports showed that CTC technology has a certain significance in the early diagnosis of PCa, but its main value is demonstrated in drug sensitivity and prognosis evaluation in the late stage of the malignancy. The standardized detection methods and reference values of CTCs in PCa will be important research orientations in the near future.

Retrospective Analysis of Single Closed Chest Drainage in Superior Lobectomy of Lung Cancer / 中国肺癌杂志

BACKGROUND@#There is no consensus on one or two chest drains closed chest drainage in superior lobectomy of lung cancer and mediastinal lymph node dissection. This study investigated the postoperative complications about drainage and evaluated the effectiveness.@*METHODS@#We retrospectively reviewed the clinical data of patients with single closed chest drainage in superior lobectomy of lung cancer and mediastinal lymph node dissection of Beijing Friendship Hospital between April 2012 and May 2017, and evaluated the effectiveness.@*RESULTS@#From the available data of 301 patients, the complication rate after superior lobectomy was 9.3%, and the complication rate of drainage after superior lobectomy was 5.64%.@*CONCLUSIONS@#The effectiveness of drainage of single closed chest drainage in superior lobectomy of lung cancer and mediastinal lymph node dissection is no less than double drainage.

Chemokine Receptor CXCR3 in the Spinal Cord Contributes to Chronic Itch in Mice / 神经科学通报·英文版

Recent studies have shown that the chemokine receptor CXCR3 and its ligand CXCL10 in the dorsal root ganglion mediate itch in experimental allergic contact dermatitis (ACD). CXCR3 in the spinal cord also contributes to the maintenance of neuropathic pain. However, whether spinal CXCR3 is involved in acute or chronic itch remains unclear. Here, we report that Cxcr3 mice showed normal scratching in acute itch models but reduced scratching in chronic itch models of dry skin and ACD. In contrast, both formalin-induced acute pain and complete Freund's adjuvant-induced chronic inflammatory pain were reduced in Cxcr3 mice. In addition, the expression of CXCR3 and CXCL10 was increased in the spinal cord in the dry skin model induced by acetone and diethyl ether followed by water (AEW). Intrathecal injection of a CXCR3 antagonist alleviated AEW-induced itch. Furthermore, touch-elicited itch (alloknesis) after compound 48/80 or AEW treatment was suppressed in Cxcr3 mice. Finally, AEW-induced astrocyte activation was inhibited in Cxcr3 mice. Taken together, these data suggest that spinal CXCR3 mediates chronic itch and alloknesis, and targeting CXCR3 may provide effective treatment for chronic pruritus.

Gait characteristics of patients with sciatica / 医用生物力学

Objective To analyze and summarize the gait characteristics of patients with sciatica, so as to assist with diagnosis and evaluation for such patients in clinic. Methods Forty-three patients with lumbar disc herniation accompanied by siatica were fitted with portable gait analyzer, and required to walk at the self-selected comfortable speed for a distance of 120 m. Forty-three healthy subjects with matched age, gender and body mass index (BMI) were recruited as control. The gait data including 7 spatial-temporal parameters (single-support duration, double-support duration, ratio of single-support duration to double-support duration, duration of gait cycle, step speed, step frequency, step length) and 4 acceleration parameters (pulling acceleration, swing power, ground impact, foot fall) were collected to compare the gait differences between patients and healthy subjects, as well as between affected and healthy limbs of patients. Results The single-support duration, ratio of single-support duration to double-support duration, step speed, step frequency, step length and four acceleration parameters of patients with sciatica were obviously smaller than those of healthy subjects, while the double-support duration of patients with sciatica was increased. The affected limb of patients with sciatica showed a significant decrease in single-support duration, step frequency and all four acceleration parameters but increase in step length as compared to their healthy limbs. Conclusions Patients with sciatica have significant gait abnormalities due to their affected limbs, which influence their walking ability. Portable gait analyzer can be used for objectively characterizing the walking abnormalities of patients, so as to provide additional information for the clinical diagnosis and evaluation.

Organic erectile dysfunction and metabolic syndrome in young and middle-aged men: analysis of 154 cases / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the association of erectile dysfunction (ED) with metabolic syndrome ( MS) and the total testosterone ( tT) level in young and middle-aged men.</p><p><b>METHODS</b>This study included 154 organic ED outpatients aged 20 -59 years and 103 age-matched men with normal sexual life. We obtained their waist circumference ( WC) , blood pressure ( BP) , fasting blood glucose ( FBG) , triglyceride ( TG) , high density lipoprotein ( HDL) , tT, IIEF -5 score, erectile function indexes and other relevant variables, and compared them between the ED and non-ED groups as well as between the ED patients with MS and those without MS.</p><p><b>RESULTS</b>The prevalence of MS was significantly higher in the ED patients than in the non-ED males ( 40. 9 vs 17.4%, P < 0. 01). Statistically significant differences were found between the ED and non-ED groups in WC ( [90. 80 ±4. 90] vs [87. 70 ±4. 60] em, P <0.01), SBP ([134.40±14.40] vs [129.90±12.40] mmHg, P<O.OS), DBP ([86.20±7.80] vs [83.60±8.60] mmHg, P <0.05), FBG ( [5.48 ±1.02] vs [5. 11 ±0. 91] mmol, P <0. 01), HDL ( [ 1.12 ±0. 27] vs [ 1. 26 ±0. 29] mmol, P < 0. 01) , and tT ( [ 15. 98 ± 6. 55] vs [ 17. 95 ± 7. 39] mmol, P < 0. 05) , as well as between the ED patients with MS and those without MS in such erectile function indexes as tT ( [ 14.49 ±6. 23] vs [ 17.01 ±6. 60] mmol, P <0. 05), erectile penile circumference ( [9. 67 ± 1.09] vs [10.16 ± 1.01] cm, P <0. 01), erectile penile length ( [ 10.40 ± 1. 20] vs [ 10.79 ± 1. 07] cm, P <0. 05), glans temperature ( [ 31. 33 ± 0. 65] vs [ 31. 80 ± 0. 82] °C , P < 0. 01) , and mean axial hardness of the penis ( [ 332. 60 ± 137. 90] vs [ 419. 20 ± 145. 80] g, P < 0. 01 ) . WC was significantly correlated with the incidence of ED ( P < 0. 01 ) .</p><p><b>CONCLUSION</b>The prevalence of MS is significantly higher in young and middle-aged ED patients than in normal males, and MS is associated with a lower testosterone level and poorer erectile performance. Central obesity is closely correlated with ED in young and middle-aged men.</p>

Urinary prostaglandins E2 correlates to overactive bladder symptoms in patients with benign prostatic hyperplasia / 中华男科学杂志

<p><b>OBJECTIVE</b>To measure the levels of urinary prostaglandins E2 (PGE2) in benign prostatic hyperplasia (BPH) patients with or without overactive bladder (OAB) symptoms and determine whether urinary PGE2 can serve as a biomarker for BPH-related OAB.</p><p><b>METHODS</b>This study included 86 BPH patients and 34 male control subjects without lower urinary tract symptoms. Based on the OAB symptom scores (OABSS), the BPH cases were classified as BPH/OAB (n =49) and BPH/non-OAB (n = 37) to be treated orally with tamsulosin alone and tamsulosin + tolterodine-tartrate, respectively, for 12 weeks. We measured the urinary PGE2 levels of all the subjects by ELISA before and after medication, the total PGE2 level normalized to the concentration of the urinary creatinine (PGE2/Cr). We also obtained the residual urine volume, Qmax, prostate volume, PSA level, IPSS and OABSS of the BPH patients, and compared them among different groups.</p><p><b>RESULTS</b>The baseline PGE2/Cr level was significantly lower in the control than in the BPH/OAB and BPH/non-OAB groups (both P <0.05), and higher in the BPH/OAB than in the BPH/non-OAB patients (P <0.05). After 12 weeks'treatment, the urinary PGE2/Cr level was remarkably decreased with relief of the OAB symptoms in the BPH/OAB patients (P <0.05) , but not in the BPH/non-OAB group (P >0.05). The concentration of PGE2 was not correlated with the IPSS storage score and OABSS of the BPH/OAB patients (P >0.05).</p><p><b>CONCLUSION</b>Patients with BPH/OAB have significantly higher urinary PGE2/Cr levels than those with BPH/non-OAB and normal controls, which tend to decrease with the alleviation of OAB symptoms. Our findings suggest that urinary PGE2 can be a potential biomarker for BPH/OAB.</p>

Clinical effect of the concentrated suture fixation method on skin transplantation in the jaw and neck region / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the clinical effect of the concentrated suture fixation method on skin transplantation on deep burn wound or wound of cicatricial deformity after burn in the jaw and neck region.</p><p><b>METHODS</b>One hundred and fourteen patients, hospitalized from April 2002 to December 2011, with deep burn or cicatricial deformity after burn in the jaw and neck region, were divided into packaging group and concentrated suture group according to the random number table. Each group had 57 patients including 48 cases with deep burn and 9 cases with cicatricial deformity. Traditional suture-package fixation method and concentrated suture fixation method were respectively used in packaging group and concentrated suture group to fix the autologous medium split-thickness skin in transplantation on wounds or scars. On post operation day (POD) 14, the skin microcirculatory perfusion flow of skin graft was measured, and the occurrence of ecchymoma, infection, and necrosis of skin in operative region were observed. The elasticity and contracture of grafted skin and scar hyperplasia on wound edge were observed 6 months after operation. Measurement data were processed with u test, while enumeration data with Fisher's exact test or Chi-square test.</p><p><b>RESULTS</b>(1) On POD 14, the skin microcirculatory perfusion flow in concentrated suture group [(2.86 +/- 0.8) V] was significantly higher than that in packaging group [(2.33 +/- 0.15) V, u = 17.776, P < 0.05]. (2) Ecchymoma occurred in 4 patients of packaging group and 3 patients of concentrated suture group, but the difference between two groups was not statistically significant (chi 2 = 0.152, P > 0.05). (3) Infection in operative region was observed in 1 patient of packaging group, while no patient in concentrated suture group showed this symptom. The difference between two groups was not statistically significant (P > 0.05). (4) Grafted skin in 6 patients of packaging group showed foliated necrosis, which was not observed on those of patients in concentrated suture group. The difference between two groups was statistically significant (P < 0.05). (5) Centipede leg-like scar hyperplasia on wound edge occurred in 21 patients in packaging group and 6 patients in concentrated suture group, and the difference between two groups was statistically significant (chi 2 = 10.920, P < 0.05). (6) Poor elasticity of grafted skin was detected in 17 patients of packaging group and 4 patients of concentrated suture group, and the difference between two groups was statistically significant (chi 2 = 9.865, P < 0.05). (7) Obvious contracture of grafted skin was observed in 15 patients of packaging group and 4 patients of concentrated suture group, and the difference between two groups was statistically significant (chi 2 = 11.684, P < 0.05).</p><p><b>CONCLUSIONS</b>Concentrated suture fixation method is suitable for application in transplantation of big sheet skin on wound in the jaw and neck region. It has high survival rate and is convenient for postoperative observation.</p>

Dual fluoroscopic image study on in vivo kinematics of cervical spine / 医用生物力学

Objective To observe the obstacle of mandible to the cervical spine with the aid of dual fluoroscopic imaging system, explore the optimal perspective Methods of in vivo kinematics of cervical spine, and verify the feasibility of cervical in vivo kinematic researches. Methods A dual fluoroscopic imaging system composed of two C-arms placed in different angles was utilized. X ray images of the cervical spine for five healthy volunteers (4 male, 1 female) with standing, flexion extension, twisting and bending positions were obtained with the C-arms in the angle of 90°, 60°and 45°, respectively. The obstacles of mandible to the cervical spine in different positions were compared and the obstacle degree of mandible to the cervical spine was evaluated to screen the optimal perspective Methods. The perspective images under conventional head rotation and coaxial trunk rotation were collected during cervical twisting to compare the obstacle of mandible to cervical images. Results There were significant differences in mandibular obstacle degrees among the three angles measured in standing, flexion-extension, twisting and bending positions（P<0.05）. With two C-arms in 45°, the cervical spine was best imaged with the least obstacle. With two C-arms in 60°, significant differences could be found between the coaxial trunk rotation and the head rotation group. Conclusions For in vivo kinematics study of cervical spine, the obstacle of mandible to the cervical spine can be minimized with C-arms of dual fluoroscopic imaging system in 45°, and the coaxial trunk rotation can decrease the mandibular obstacle degrees as compared with the head rotation, which satisfy the requirement of 2D-3D image matching.

Assessing validation of dual fluoroscopic image matching method for measurement of in vivo spine kinematics / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Accurate knowledge of the spinal structural functions is critical to understand the biomechanical factors that affect spinal pathology. Many studies have investigated the human vertebral motion both in vitro and in vivo. However, determination of in vivo motion of the vertebrae under physiologic loading conditions remains a challenge in biomedical engineering because of the limitations of current technology and the complicated anatomy of the spine.</p><p><b>METHODS</b>For in vitro validation, a human lumbar specimen was imbedded with steel beads and moved to a known distance by an universal testing machine (UTM). The dual fluoroscopic system was used to capture the spine motion and reproduce the moving distance. For in vivo validation, a living subject moved the spine in various positions while bearing weight. The fluoroscopes were used to reproduce the in vivo spine positions 5 times. The standard deviations in translation and orientation of the five measurements were used to evaluate the repeatability of technique. The accuracy of vertebral outline matching with metallic marks matching technology was compared.</p><p><b>RESULTS</b>The translation positions of the human lumbar specimen could be determined with a mean accuracy less than 0.35 mm and a mean repeatability 0.36 mm for the image matching technique. The repeatability of the method in reproducing in vivo human spine six degrees of freedom (6DOF) kinematics was less than 0.43 mm in translation and less than 0.65° in rotation. The accuracy of metallic marks and vertebral outline matching did not show significant difference.</p><p><b>CONCLUSIONS</b>Combining a dual fluoroscopic and computerized tomography imaging technique was accurate and reproduceable for noninvasive measurement of spine vertebral motion. The vertebral outline matching technique could be a useful technique for matching of vertebral positions and orientations which can evaluate and improve the efficacy of the various surgical treatments.</p>

Clinical observation on post-stroke shoulder pain treated with balance acupuncture / 中国针灸

<p><b>OBJECTIVE</b>To observe the difference of therapeutic effect on post-stroke shoulder pain between balance acupuncture and conventional acupuncture.</p><p><b>METHODS</b>Sixty cases were randomly divided into two groups, 30 cases in each one. In balance acupuncture group, the balance technique of acupuncture was used on Jiantong point. In conventional acupuncture group, the conventional acupuncture was applied to Shuigou (GV 26), Neiguan (PC 6), Tongli (HT 5), Zusanlti (ST 36), Xuanzhong (GB 39) and others, combined with the points on the affected meridians. The Visual Analogue Scale (VAS) was adopted to evaluate the efficacy.</p><p><b>RESULTS</b>Separately, before and after the 1st treatment, VAS scores were 5.62 +/- 0.46 and 5.00 +/- 0.31 in balance acupuncture group (P < 0.01), and those were 5.52 +/- 0.65 and 5.22 +/- 0.29 in conventional acupuncture group (P < 0.05). The improvement in balance acupuncture group was superior to that in conventional acupuncture group (P < 0.05). After 10 treatments (one course of treatment), VAS score was 3.75 +/- 0.67 in balance acupuncture group and was 3.77 +/- 0.62 in conventional acupuncture group, which reduced significantly as compared with those before treatment (both P < 0.01), the difference between two groups was not significant (P > 0.05).</p><p><b>CONCLUSION</b>Either balance acupuncture or conventional acupuncture has significant analgesia on post-stroke shoulder pain. But, the instant analgesia of balance acupuncture is superior to that of conventional acupuncture.</p>

Human cytomegalovirus inhibits the differentiation of human hippocampus neural stem cells / 病毒学报

The objective of present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural stem cells NSCs differentiation in vitro, Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. Immunofluorescence method was used to detect the expression of NSCs marker-Nestin within these cells. Cultured in 10% FBS, NSCs began to differentiate. On the onset of the differentiation, HCMV AD169 (MOI=5) was added into the differentiation medium. After 7 days differentiation, the effect of HCMV infection on NSCs differentiation was observed by detecting the rate of nestin, GFAP and HCMV immediate-early (IE) positive cells with confocal microscopy and immunofluorescence method. The resucts showed most of the cells (passage 4-6 ) were Nestin positive and could differentiate into NSE-positive neurons and GFAP-positive astrocytes. On day 7 postinfection, 86% +/- 12% of infected cells were IE positive. The percentage of Nestin-positive cells was 50% +/- 19% and 93% +/- 10% (t= 6.03, P<0.01)and those of GFAP-positive cells was 81% +/- 11% and 55 +/- 17% (t=3.77, P<0.01) in uninfected and infected cells respectively. These findings indicated that NSCs were HCMV permissive cell and HCMV AD 169 infection suppressed the differentiation of Hippocampus-genetic human neural stem cells into astrocytes.

The expression and anti-apoptotic function of HCMV IE2 protein controlled by Tet-On system / 病毒学报

During the infection of host cells, IE2 protein is one of the first and most abundantly expressed products of HCMV genome, which plays an important role in the controlling of cell cycle and apoptosis. But the correlation between expression level and anti-apoptotic activity of IE2 protein is still not clear. In this study, we successfully established a HCMV IE2 protein expression cell line that was controlled by Tet-On system. The effect of IE2 protein on cell apoptosis and the expression of p53 was detected under different condition of induction. Our results showed that the IE2 protein could inhibit cell apoptosis induced by TNF-alpha. Additionally, the anti-apoptotic activity of IE2 protein seemed to be relevant to its expression level. However, we failed to detect any difference of p53 expression between the IE2 protein expression and non-expression cells. These data indicated that the IE2 protein might inhibit cell apoptosis through regulating different signal pathways.

Dynamic change in microcirculation of pancreas after experimental high-voltage electric burn / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the changes in surface microcirculation of pancreas after high-voltage electric burn (HEB).</p><p><b>METHODS</b>Thirty rabbits were divided into electrical injury (E) group and control (C) group in a simple random method, with 15 rabbits in each group. Rabbit model of HEB was reproduced from E group with TC-30-20KVA type voltage regulator and YDJ-10KVA type experimental transformer. Rabbits in C group were shamly burned with the same equipment as in E group but not electrified. Intravenous blood of rabbits in both groups was drawn 15 mins before HEB and 0, 1, 2, 4, 8 h after to determine the levels of serum amylase and blood glucose. The morphology of the pancreas microvessels and its surrounding tissues, and the dynamic changes in microvascular blood flow were observed with WX-9 microscope and its image analytical system.</p><p><b>RESULTS</b>The level of serum amylase of rabbits in E group increased gradually and peaked (849 +/- 39) U/L at 8 post HEB h (PHH), which decreased gradually reaching the nadir (153 +/- 21) U/L at 8 PHH in C group (P < 0.05). The blood glucose levels of rabbits in E group and C group increased gradually, with the former level obviously higher than the latter (P < 0.05). Arteriole, venule and capillary network on the surface of pancreatic lobules of rabbits in both groups were clearly seen and well-distributed in the natural way before HEB. In E group, arterioles of rabbits contracted at 0 PHH, and increased gradually in caliber size at 1 PHH; venules of rabbits were unevenly thickened at 2 PHH, and dilated at 8 PHH; the capillaries were contracted or with interrupted flow or completely obstructed at 0 PHH, and their thickness were uneven at 2 PHH, showing exudation at 8 PHH. There was no obvious change of microvessels in rabbits in C group at each time point. There was no exudation and bleeding around the microvessels on the pancreas surface of rabbits in both groups before HEB. In E group exudation was observed around microvessels at 1 PHH, bleeding was observed at 2 PHH and became obvious at 4 PHH; exudation and diffuse bleeding from capillaries were observed at 8 PHH. There was no exudation and bleeding in rabbits in C group as observed at each time point. Before HEB, blood flow speed in microvessels of rabbits in 2 groups was similar to each other (P > 0.05), and no erythrocyte aggregation or microthrombus was found in both groups. In E group, blood flow speed slowed down at 0 PHH as compared with that before HEB, it accelerated at 1 h and slowed down later; erythrocyte aggregation in venules and capillaries was found at 0 PHH, and it aggregated gradually. No above-mentioned change was found in C group.</p><p><b>CONCLUSIONS</b>HEB produces microcirculation disturbance and functional disturbance of pancreas.</p>

Effect of human cytomegalovirus infection on nerve growth factor expression in human glioma U251 cells / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV).</p><p><b>METHODS</b>U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV.</p><p><b>RESULTS</b>The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P < 0.05).</p><p><b>CONCLUSION</b>HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.</p>

Electron microscopic observation of Randall's plaque in patients with calcium oxalate stone / 中华泌尿外科杂志

Objective To study the ultrastructure of the renal papillary Randall's plaque in calclum oxalate stone formers. Methods The 14 biopsy samples of the Randall's plaque in 12 patients with calcium oxalate stone undergoing PCNL for stone removal were obtained using endoscopic biopsy technique,followed by staining with hematoxylin-eosin or fixing with osmium tetroxide,and then the ultrastructure of the Randall's plaque was observed under light microscope and transmission electron microscope. Results In all 12 patients,72 renal papillae were examined.All kidReys were found to have papillary plaque and 7 of the patients had attached stones.Sixty-three papillae(87.5%)contained plaque.Calcium deposition was seen in the 12 renal papilla tissue by light microscopy.Transmission electron microscopy images of the 2 Randall's plaque samples showed several cluster of sharp and large crystals lied closer to the surface of Randall's plaque.The typical crystals were acicular with light profile. Conclusions Randall's plaque is an interstitial medullary and papillary deposit of calcium oxalate.The appearance of the deposition of calcium oxalate crystals lies upon Randall's plaque,which might be an explanation for the mechanism of calcium oxalate stone formation.

Relationship between HER-2/neu over-expression and androgen independent prostate cancer / 中华男科学杂志

<p><b>OBJECTIVE</b>To detect HER-2/neu expression in prostate cancer tissues of both androgen dependent and independent groups and to evaluate the role of HER-2/neu in androgen independent prostate cancer.</p><p><b>METHODS</b>Immunohistochemical assay was used in the detection of HER-2/neu in the prostate cancer samples from 30 cases of androgen dependent cancer and 24 cases of androgen independent cancer. The correlation was analyzed between HER-2/neu over-expression and the tumor's clinical stage and Gleason score.</p><p><b>RESULTS</b>The rates of HER-2/neu over-expression were 10% and 33% in the androgen dependent group and the androgen independent group, significantly higher in the Gleason score >7 group and the clinical stage > T2 group than in the -7 group and the > T2 group (14.29% vs. 26.92%, 34.62% vs. 7.14%, P < 0.01).</p><p><b>CONCLUSION</b>The rate of HER-2/neu over-expression is high in androgen independent prostate cancer and is correlated with the tumor stage and Gleason score.</p>

Using the SELDI Protein Chip System to Detect Changes in Protein Expression in Vero Cells after Infection / 中国病毒学

Human herpes simplex virus 1 (HSV-1) causes facial,ocular,and encephalitic disease and is associated with latent infection and cancer.Here,we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein expression in Vero cells cultured in vitro.After infection with HSV-1 and culture for 12,24 or 48 h,cells were harvested and lysed.IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences before and after infection.The chip detected a series of differentially expressed protein peaks.Interestingly,both peaks at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG 15,which may participate in antiviral activity during the process of infection.Thus,the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and the interaction between the virus and its host.In addition,they can help in the discovery of new therapeutic targets for treatment of HSV-1 infection.

Analysis of diagnosis and treatment of 66 cases of testicular torsion / 上海第二医科大学学报

Objective To analyse the diagnosis and treatment of testicular torsion. Methods The clinical data of 66 cases of testicular torsion were retrospectively analysed. Results Among the 66 cases,32(48.5%) paid the first medical visit within 10 h,and 24(36.4%)were confirmed diagnosis at the first visit.False negative results occurred with color Doppler flow imaging(CDFI),and 8 testicles were damaged due to the false negative diagnosis.Thirty-three patients without prophylactic contralateral orchidopexy were followed up for 6 months to 20 years,and one experienced recurrent torsion. Conclusion The testicular torsion must be considered when a sudden acute scrotum pain is occurred.Testicular damage is closely related to the torsion time,and delayed medical intervention contributes to the testicular damage.Highly suspected cases should be performed surgical exploration timely due to the false negative results with CDFI.Prophylactic contralateral orchidopexy is recommended.