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Objective To investigate the relationship between ORMDL3/GSDMB polymorphism and genetic susceptibility to childhood asthma.Methods The electronic databases PubMed,Wanfang,China National Knowledge Infrastructure (CNKI),Weipu,and China Biology Medicinedisc (CBM) were browsed for published case-control studies on investigating the association between ORMDL3/GSDMB polymorphism and genetic susceptibility to childhood asthma.Odds ratio(OR) and 95％ confidence interval (95％ CI) were used to investigate the strength of the association.Fixed or random effect models were selected for pooled OR calculation.Publication bias was assessed.All statistical analysis was conducted with Review Manager 5.0 software.Results Six case-control studies with a total of 3289 childhood asthma cases and 3391 controls were included.For the homozygote TT and T allele carriers (TT + TC),the pooled ORs (95％ CI) were 1.86 (1.58-2.20) and 1.56 (1.35-1.80) compared to the homozygous genotype (CC).In the stratified analysis by ethnicity,the ORs (95％ CI) of the T allele carriers and the homozygote TT were 1.50(1.15-1.96) and 1.51 (1.15-1.98) among Asians.While among European,the ORs (95％ CI) of the T allele carriers and the homozygote TT were 1.69 (1.42-2.02) and 2.11 (1.71-2.61).Conclusions ORMDL3/GSDMB polymorphism is overall associated with childhood asthma susceptibility.However,the susceptibility in the Asians is a little lower compared with that of the European populations,which suggest a possible role of ethnic differences in genetic backgrounds.
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Objective To compare the inhibition effect of budesonide suspension and dexamethasone on the rats′ pneumonic anaphylacticitis and serum IL-5,and study the effect mechanism in treatment of asthma with glucocorticoids on systemic administration and with budesonide suspension.Methods Twenty-six health wistar rats were randomly divided into normal group,asthmatic group,budesonide suspension group and dexamethasone group.Asthma models were sensitized with egg albumin.Before sensitized,budesonide suspension group were administered inhalation of budesonide suspension,and dexamethasone group were administered abdominal injection of dexamethasone.The level of IL-5 was measured with enzyme linked immunosorbent assay(ELISA) reagent box,lung was eviscera-(ted after collecting) blood,pathological slice was made.Results The rats′ pathological changes of lung and bronchus were significant between asthma group and other groups,while there were no significant difference between budesonide suspension group and dexamethasone group,but both of two groups showed slight difference with normal group.Compared with normal group,the level of serum IL-5 in asthma group raised obviously,and there was significant difference(P0.05).Conclusions The treatment of inhalation of budesonide suspension can inhibit rats′ pneumonic anaphylacticitis,ameliorate rat anaphylactic asthma symptom.
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Objective To explore the inhibition effect of interleukin-10(IL-10)on pneumonic anaphylacticitis in mice with asthma and its effects on the level of serum IgE.Methods Thirty BALB/c male mice whose weight were(20?2)g were randomly divided into 3 groups:normal group(n=10),asthma model group(n=10)and IL-10 treatment group(n=10).Asthma model group and IL-10 group were sensitized with 1 g?L-1 ovalbumin,then asthma model was made with inhallating 10 g?L-1 VOA 20 mL.The normal group was not administered anything as a negative group.Before inhallating with 10 g?L-1 VOA,asthma model group was administered inhalation with 9 g?L-1 saline 0.1 mL,and IL-10 treatment group was administered inhalation with 0.1 mL IL-10 whose concentration was 0.2 ?g?L-1.The level of IgE was measured by ELISA reagent box,eviscerated lungs after collecting blood and then made them into pathological slices.Results There was an obvious distinction in the mice pathological change of lungs and bronchus between asthma model group and other groups,but no significant difference between IL-10 treatment group and normal group.There was no obvious infiltration of inflammatory cells in the airways of IL-10 treatment group and normal group.However,there were a great number of inflammatory cells in the interstitial and peribronchovascular regions of asthma model group.The levels of serum IgE were(5.219?0.427)?g?L-1,(6.633?0.145)?g?L-1 and(5.503?0.566)?g?L-1 in normal group,asthma model group and IL-10 treatment group,respectively.The level of serum IgE in asthma model group increased obviously compared with normal group and IL-10 treatment group,with significant differences(Pa0.05).Conclusions Inhalation of IL-10 can optimally control the pneumonic anaphylacticitis,improve the situation of airway inflammation in mice asthmatic model and reduce the IgE serum level.
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Objective To discuss the level and its relativity analysis of IL-16 and IgE in asthmatic children .Methods Compare and analyze the level of IL-16,IL-10 and IgE between 18 asthmatic children and 14 healthy children.Results The serum level of IL-16 and IgE in asthmatic children was much higher than the contrast, P
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Objective To explore the changes of the levels of interleukin-17(IL-17),matrix metalloproteinases-9(MMP-9) and immunoglobulin E(IgE) in serum of asthmatic children and analyzing the correlation.Methods Twenty-eight asthmatic children and 14 healthy children were collected to determin the levels of IL-17,MMP-9 by sandwich enzyme linked immunosorbent assay,and the correlation of IL-17,MMP-9 and IgE in serum were analyzed.Results The levels of IL-17,MMP-9 and total IgE in serum of asthmatic children were significantly higher than that of control group(Pa0.05).Conclusions The levels of IL-17 and MMP-9 increase in asthmatic airway inflammation.IL-17 and MMP-9 play an important role in asthmatic airway inflammation and airway rebuilding.
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Objective To evaluate the changes of peripheral blood interleukin(IL) 2, IL 10, IL 13 in the serum of asthmatic children and their effect on the pathogenesis of asthma.Method The serum levels of IL 2,IL 10,II 13 in 16 cases of asthmatic children and 10 cases of healthy controls were measured by ELISA methods.Results The serum levels of IL 2,IL 13 in asthmatic patients were significantly higher than those in healthy controls( P