RESUMO
AIM To study the effects of Zishen Yutai Pills (Codonopsis Radix,Dipsaci Radix,Morindae officinalis Radix,etc.) on endocrine in diminished ovarian reserve (DOR) rats.METHODS Female SD rats with normal oestrous cycle were induced by intragastric administration of 55 mg/(kg · d) tripterygium glycosides for fourteen days to establish DOR model,and then divided into Zishen Yutai Pills high-,low-dose groups,Bujiale group,model control group and negative control group.After 35 days' administration,serum estradiol (E2),follicle-stimulating hormone (FSH),luteinizing hormone (LH) and progesterone (P) levels were detected by ELISA.And ovarian tissue estrogen receptor (ER),follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) expressions were detected by immunohistochemical method.RESULTS Compared with the negative control group,there were significant changes in levels of E2,FSH,LH,P,and expressions of ER,FSHR,LHR in the model control group (P ＜ 0.05),but there were no statistical significances in body weight,ovary index,uterus index and serum FSH/LH level between the two groups (P ＞ 0.05).Compared with the model control group,E2 level was increased in the Zishen Yutai Pills high-,low-dose groups and Bujiale group,FSH,LH levels were decreased,and they also promoted ER expression and restrained FSHR,LHR excessive expressions,which were statistically significant (P ＜ 0.05).P level was significangly increased in different dose groups (P ＜ 0.05),FSH/LH in the high-dose group was obviously decreased (P ＜ 0.05),and body weight,ovary index,uterus index in various intervening groups showed no statistical significances (P ＞ 0.05).CONCLUSION Zishen Yutai Pills can improve ovarian reserve function in DOR rats by affecting the expression of ovarian tissue hormone receptor and regulating endocrine function.