RESUMO
Objective To study the effect of over-expression of KLF 4 on the proliferation and migration ability of K562 cells.Methods The K562 cells with KLF4 over expression ( K562-KLF4 cells) were used as experimental group, and paralleled with the vector control ( K562-C1 cells) and blank K562 cell control.Real-time PCR was performed to detect the relative expression quantity of KLF4 mRNA of each group cells respectively;Western blot was performed to detect the level of KLF 4 protein of each group cells respectively .The cell proliferation was tested by MTS assay.The migration ability of K562 cells was detected by Transwell .Results Compared with K562-C1 cells and blank K562 cells, the relative expression of KLF4 mRNA of K562-KLF4 cells was significantly increased (P<0.05).The level of KLF4 protein of K562-KLF4 cells was also significantly increased, by 77.78%(P<0.05).The proliferation ability and migration ability of the K 562 cells with over-expressing KLF4 were inhibited sig-nificantly (P<0.05).Conclusions Over-expression of KLF4 could inhibit the proliferation and migration ability of K562 cells.
RESUMO
We investigated the characteristics and epidemiological characteristics of severe fever with thrombocytopenia syndrome (SFTS) infection in Tiantai County,Zhejiang Province.Active surveillance and epidemiological investigation were employed with the serum samples collected simultaneously.The samples were detected by fluorescence quantitative PCR assay recommended by national standard of China.By descriptive epidemiology,the SFTS cases in 2012-2015 in Tiantai and related data were analyzed.In 2012-2015,there were 12 new cases of SFTS infection and 2 deaths,including 5 males and 7 females (39 to 75 yrs).The peak incidence was found from April to August.The main clinical manifestations was fever (100 ％),chills and fatigue (83％),and body pain (75％).The positive rate of nucleic acid screened in subclinical population was 1.4％;8 ca ses with definite records of tick bites (67 ％).In conclusion,several cases of SFTS have been confirmed in Tiantai County with two inapparent infections,which suggest the urgency and importance to strengthen the active surveillance,prevention and control measures and health education s in the coming days.
RESUMO
We investigated the characteristics and epidemiological characteristics of severe fever with thrombocytopenia syndrome (SFTS) infection in Tiantai County,Zhejiang Province.Active surveillance and epidemiological investigation were employed with the serum samples collected simultaneously.The samples were detected by fluorescence quantitative PCR assay recommended by national standard of China.By descriptive epidemiology,the SFTS cases in 2012-2015 in Tiantai and related data were analyzed.In 2012-2015,there were 12 new cases of SFTS infection and 2 deaths,including 5 males and 7 females (39 to 75 yrs).The peak incidence was found from April to August.The main clinical manifestations was fever (100 ％),chills and fatigue (83％),and body pain (75％).The positive rate of nucleic acid screened in subclinical population was 1.4％;8 ca ses with definite records of tick bites (67 ％).In conclusion,several cases of SFTS have been confirmed in Tiantai County with two inapparent infections,which suggest the urgency and importance to strengthen the active surveillance,prevention and control measures and health education s in the coming days.