RESUMO
Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1,1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728,P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1,1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.
RESUMO
Objective To compare the traditional Chinese medicine enema and routine enema in the period of gastrointestinal dysfunction in patients with severe burn infection the clinical effect of treatment. Methods The control group weakened symptoms on gastrointestinal peristalsis began to appear fasting, gastrointestinal decompression, anal exhaust, saline enema, parenteral nutrition, antibiotic treatment. On the basis of routine treatment in the treatment group the enema for Chinese medicine prescription. Results The curative effect in the treatment group after enema 83.3%(30/36) compared with the control group 41.70% (15/36) had significant difference (P<0.05). At the time of borborygmus, abdominal distension disappeared time, anus Exhaust time, the treatment group were (7.0±0.8)d, (3.4±0.9)d, (7.3±0.8)d, the control group were (12.6±1.8)d, (7.1±1.2)d, (13.4±1.9)d, two sets of data were compared with statistical difference (P<0.05). Conclusion The traditional Chinese medicine enema for large surface gastrointestinal dysfunction during infection deposition burn patients is rapid, safe and reliable, convenient administration, significant curative effect on gastrointestinal functions in patients with motility.
RESUMO
Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1,1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728,P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1,1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.
RESUMO
Objective To compare the traditional Chinese medicine enema and routine enema in the period of gastrointestinal dysfunction in patients with severe burn infection the clinical effect of treatment. Methods The control group weakened symptoms on gastrointestinal peristalsis began to appear fasting, gastrointestinal decompression, anal exhaust, saline enema, parenteral nutrition, antibiotic treatment. On the basis of routine treatment in the treatment group the enema for Chinese medicine prescription. Results The curative effect in the treatment group after enema 83.3%(30/36) compared with the control group 41.70% (15/36) had significant difference (P<0.05). At the time of borborygmus, abdominal distension disappeared time, anus Exhaust time, the treatment group were (7.0±0.8)d, (3.4±0.9)d, (7.3±0.8)d, the control group were (12.6±1.8)d, (7.1±1.2)d, (13.4±1.9)d, two sets of data were compared with statistical difference (P<0.05). Conclusion The traditional Chinese medicine enema for large surface gastrointestinal dysfunction during infection deposition burn patients is rapid, safe and reliable, convenient administration, significant curative effect on gastrointestinal functions in patients with motility.