RESUMO
Objective To carry out the quantitative analysis of the injury degree of model rat of doxorubicin-induced nephropathy based on metabolomics. Methods 1H-NMR based metabolomics approach combined with multivariate statistical analysis were used to analyze rat serum samples from different doses and different times of doxorubicin administration and identify the progressive markers reflecting the progress degree of nephropathy. Moreover, the evaluation of injury degree of rat nephropathy model induced by doxorubicin was conducted according to the change degree of related markers. Results Nine potential biomarkers for pathological conditions were obtained such as 2-oxoisocaproate, isoleucine, leucine, valine, pyruvate, 3-hydroxybutyric acid, citric acid, creatinine, and carnitine, among which 2-oxoisocaproate and carnitine were in a dose-dependent manner, that is, the greater the dose of doxorubicin, the greater the variation of the endogenous metabolites, the more severe kidney injury, indicating that 2-oxoisocaproate and carnitine can be used as a progressive markers reflecting the process of kidney disease. Conclusion This study provides the diagnostic marker of nephropathy, and provides research methods and ideas for the study of other progressive diseases and the discovery of early diagnostic markers.