Effect of exogenous 5-aminolevulinic acid on seed germination and antioxidase activities of Perilla frutescens seedlings under NaCl stress / 中草药

Objective: In order to get the method of improving the salt resistance of seeds and seedlings for Perilla frutescens under NaCl stress, seed germination and physiological characteristics of P. frutescens seedlings were studied. Methods: Several physiological indexes of P. frutescens seeds treated by 5-aminolevulinic acid (5-ALA) were measured. And other indexes of the seedlings like relative water content (RWC), the biomass, the content of soluble sugar, and malondialdehyde (MDA), the activities of superoxide (SOD), peroxidase (POD), and catalase (CAT) were also measured. Results: The germination indexes of P. frutescens seeds under NaCl stress have a obvious inhibition. But after the treatment at different concentrations of 5-ALA every germination index were all increased. And the seeds treated by 5-ALA with the concentration of 50 mg/L have the most significantly increase in every index. The germination vigor was 71.3%, the germination rate was 90.5%, the germination index and vigor index were 15.9 and 0.129 6, respectively. Every treatment of them coule improve the biomass and soluble sugar of the seedlings; The RWC of P. frutescens leaves decreased under NaCl stress, but after treated by 5-ALA, the rate of decrease has been relieved and the content of MDA in leaves was decreased. The activities of three enzymes including SOD, POD, and CAT were all increased. And the treatment of 5-ALA with the concentration of 50 mg/L got the maximin with 0.79, 7.69, and 5.84 U/mg, respectively. Conclusion: 5-ALA with the appropriate concentration of 50 mg/L could significantly alleviate the damages to the seeds and seedlings of P. frutescens under NaCl stress and promote the salt resistance of the seeds and seedlings.

Effects of photodynamic therapy with 5-aminolevulinic acid on human ovarian carcinoma 3AO cells in vitro / 西安交通大学学报(医学版)

Objective To study the effects of 5-aminolevulinic acid (5-ALA) mediated photodynamic therapy (PDT) on human ovarian carcinoma 3AO cells in vitro. Methods Morphological changes were examined by microscopy; the inhibitory effects of 5-aminolevulinic acid mediated photodynamic(5-ALA-PDT) on 3AO cells were examined by MTT assay; cell cycle of 3AO cells was observed by flow cytometry with PI staining; the modality of 3AO cell death induced by 5-ALA-PDT was determined by flow cytometry with Annexin V-PI staining. Results Typical morphological changes of apoptosis occurred after 5-ALA-PDT. The inhibition rate of cells increased gradually with the increase of 5-ALA concentration and laser irradiation dose (P