A new 8-O-4'-neolignan from twigs and leaves of Ervatamia hainanensis / 中草药

Objective: To study the chemical constituents in the twigs and leaves of Ervatamia hainanensis. Methods: The chemical constituents were isolated by silica gel, ODS, Sephadex LH-20 column chromatographies, and preparative HPLC. The structures were elucidated on the basis of physicochemical properties and spectral analysis. Results: Four lignans were isolated and identified as threo-7-O-ethylguaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (1), threo-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (2), erythro-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (3), and dehydrodiconiferyl alcohol (4). Conclusion: Compound 1 is a new 8-O-4'-neolignan and named as hainanervaligan A (1), and compounds 2-4 are obtained from the plants of Ervatamia (A. DC.) Stapf for the first time.

Chemical constituents from Spiraea pubescens / 中草药

Objective: To investigate the chemical constituents from the ethanol extract of Spiraea pubescens. Methods: The compounds were isolated and purified by chromatography on silica gel, ODS, and preparative HPLC. Their structures were elucidated on the basis of chemical and spectroscopic methods, including MS, 1D, and 2D NMR spectral techniques. Results : Fourteen compounds were isolated and identified as β-sitosterol (1), tricosyl alcohol (2), stigmast-4-en-3-one (3), pentacosyl alcohol (4), stigmastanol (5), (+)-cyclo-olivil (6), (+)-africannal (7), (+)-lyoniresinol (8), 5-methoxy-(+)-isolariciresinol (9), (+)-isolariciresinol (10), (7R,8R)-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan (11), (6S,9R)-6-hydroxy-3-one-α-ionol-9-O-β-D-glucopyranoside (12), (+)-lyoniresinol 9-O-β-D-xylopyranoside (13), and (-)-lyoniresinol 9-O-β-D-xylopyranoside (14). Conclusion: Compounds 2-5 and 7-14 are isolated from the plants of Spiraea L. for the first time, and compounds 1 and 6 are obtained from this plant for the first time.

A new 8-O-4′-neolignan from stem barks of Lindera caudata and its cytotoxicity / 中草药

Objective: To study the chemical constituents in the stem barks of Lindera caudata. Methods: The chemical constituents from the stem barks of L. caudata were isolated by column chromatographic methods of silica gel, MCI-Gel resin, Sephadex LH-20, and high performance liquid chromatography. The structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. The cytotoxicity of this compound for NB4, A549, SHSY5Y, PC3, and MCF7 cells line was also observed by using the MTT method. Results: A 8-O-4′-neolignan, caudatalignan A (1) was isolated from this plants. Conclusion: Compound 1 is a new compound and displays the cytotoxicity against NB4 and SHSY5Y cells with IC50 values of 4.2 and 5.0 μmol/L, respectively.

Chemical constituents from roots of Kadsura longipedunculata / 中草药

Objective: To study the chemical constituents from the roots of Kadsura longipedunculata. Methods: The constituents were isolated and purified by various chromatographic methods, and the structures were elucidated by spectroscopic analysis. Results: Sixteen compounds were isolated from the roots of K. longipedunculata and the structures were identified as pinobatol (1), leptolepisol B (2), 7S,8R-erythro-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan (3), 2,3-bis-(α-hydroxy-4-hydroxy-3-methoxybenzyl)-butane-1,4-diol (4), (7'S,8R,8'S)-4,4',9-trihydroxy-3,3',5-trimethoxy-9'-O-β-D-xylopyranosyl-2,7'-cyclo-lignan (5), aviculin (6), ent-isolariciresinol (7), lawsorosemarinol (8), (+)-anwulignan (9), isolariciresinol-2α-O-β-D-xyloside (10), procyanidin B3 (11), prodelphinidin B3 (12), (-)-gallocatechin (13), (+)-catechin (14), abscisic acid-β-D-glucopyranosyl ester (15), and (-)-oleuropeic acid 8-O-β-D-glucopyranoside (16). Conclusion: Compounds 1-8, 11-13, 15, and 16 are isolated from the plants of Kadsura Kaempf. ex Juss. for the first time.