Genotyping of Dombrock and Lutheran blood group systems in blood donors from the southwestern region of the state of Paraná, Southern Brazil

Abstract Background Lutheran and Dombrock are two blood group systems with low immunogenic antigens; they can cause mild-to-moderate transfusion reactions. For both, immunophenotyping is not performed in the pretransfusion routine in Brazil. In addition, the distribution of their antigenic frequencies is an important marker of ethnicity. Thus, the goal of this study was to carry out the genotyping of the LU*01, LU*02, DO*01 and DO*02 alleles of the Lutheran and Dombrock blood group systems in blood donors from the southwestern region of the state of Paraná, Southern Brazil. Method Genotyping was performed for 251 blood donors by specific allele-polymerase chain reaction. The genotype and allele frequencies were obtained through direct counting and compared with other Brazilian populations using the chi-square test with Yates correction. Results The distribution of genotype frequencies for LU were 0.4% for LU*01/LU*01, 6.8% for LU*01/LU*02 and 92.8% for LU*02/LU*02 and for DO, they were 19.9% for DO*01/DO*01, 44.6% for DO*01/DO*02 and 35.5% for DO*02/DO*02. The allele and genotype frequencies of LU and DO were similar to those expected for Caucasians, but the DO*01/DO*01 genotype frequency was different to other Brazilian populations. The rare LU*01/LU*01 genotype was found in a loyal blood donor. Conclusion The genotyping techniques allowed the evaluation of the LU*01, LU*02, DO*01 and DO*02 alleles in blood donors registered in the Hemotherapy Center of the southwestern region of Paraná, Southern Brazil, and contributed to a genotyped blood donor database.

Effects of miR-125b on proliferation and invasion of hepatocarcinoma cells by ART4 protein / 中国免疫学杂志

Objective:To investigate the effect of miR-125 b on ART4 protein and its effect on proliferation and invasion of hep-atocellular carcinoma cells .Methods:The expression of miR-125 b in hepatocellular carcinoma and hepatocellular cells was detected by qPCR.The expression of miR-125b and ART4 was detected by qPCR after overexpression of miR-125b.The expression of miR-125b and ART4 was detected by double luciferase assay .The effect of miR-125 b on the proliferation of hepatoma cells was detected by MTT assay.The effect of miR-125b on the invasion of hepatoma cells was detected by Transwell invasion assay .MTT assay was used to detect the effect of miR-125 b on the proliferation of hepatoma cells after overexpression of ART 4.Transwell invasion assay was used to detect the effect of miR-125 b on the invasion of hepatoma cells after overexpression of ART 4.Results: The expression of miR-125 b in hepatoma cells was low,and overexpression of miR-125b could inhibit the expression of ART4 protein.Overexpression of miR-125b could inhibit the proliferation and invasion of hepatoma cells .Overexpression of ART4 could reverse the proliferation and invasion of hepatoma cells by miR-125b.Conclusion:Expression of miR-125b in hepatocellular carcinoma was down-regulated.Meanwhile,miR-125 b can regulate the expression of ART 4 and affect the proliferation and invasion of hepatoma cells .