RESUMO
Objective To study the effect of Abnormal Savda Munziq (ASMq) on MDR and expression of mRNA of Bel/Fu cell line. Methods Several genes expression including MDR1, MRP, TOPOII? and GST-? were analyzed by RT-PCR. Results ASMq has been found to down-regulate MDR1 gene and simultaneously up-regulate TopoⅡ?mRNA. Conclusion ASMq can reverse the drug resistance against 5-Fu in Bel/Fu cells. Inhibition of MDR1 mRNA and up-regulate TopoⅡ? mRNA may contribute to the mechanisms.
RESUMO
Objective To investigate the reverse effect of the extracted solution of uygur medicine ASMq on the liver cancer drug-resistance cell line Bel/Fu in vitro.Methods MTT assay was used to determine the IC50 of 5-Fu on Bel/Fu cell line and the reverse multiples of the drug-resistance before and after ASMq treatment.The concentration was used to determine the fold reversal of ASMq.By clone forming text to determine the reversal effect on drug resistance of Bel/Fu cell line.Results The IC50 value of ASMq crude extract was 2 000.68 ?g/mL.ASMq at 390 ?g/mL concentration was no toxic but had a coordinate effect on Bel/Fu cell line with 5-Fu.The fold reversal of ASMq combining with 5-Fu was 7.8,CDI was 0.89.The clone forming size became smaller comply with the concentration of ASMq.Conclusion ASMq has reversal effect on drug resistance of Bel/Fu cell line.