RESUMO
Objective To investigate the reversal effect of abnormal savda munziq total flavonoids on leukemic cell lines.Methods CCK -8 method was used to detect the cytotoxicity of abnormal savda munziq total flavonoids on leukemia cells,by multivariate analysis of variance in the chemotherapy drug intervention,further calculated the reversal fold.Results CCK -8 results showed that with the increase of concentration of ASMq800(μg/mL)compared with 200(μg/mL)and 400(μg/mL),the A value of K562 /ADR cells gradually decreased,the growth curve suggested that with the increase of ASMq concentration in K562 /ADR cell,survival time was shortened,the inhibition of the cells was significantly increased.Different concentrations of ASMq on the adriamycin toxicity with increase of concen-tration of adriamycin,the inhibition of ASMq on multidrug resistance cell line was significantly higher.The reversal multiples calculated that with the increase of ASMq concentration 600 μg/mL and 900 μg/mL,the reversal index increased significantly 2.485 times and 3.651 times.Conclusion ASMq on human leukemia multidrug resistance cell line K562 /ADR could reverse the drug resistance,the reversal mechanism needs further study.
RESUMO
AIM: To study the molecular mechanisms of Abnormal Savda Munziq responsible for its anticancer property. METHODS: Anti-proliferative effect of Abnormal Savda Munziq ethanol extract was determined by MTT assay;DNA fragmentation was determined by gel electrophoresis assay,cell cycle analysis was detected by flow cytometer;apoptosis related gene expression was detected by RT-PCR assay;Caspase-3 activity was determined by Caspase-3 assay kit. RESULTS: Abnormal Savda Munziq ethanol extract possessed inhibition effect on HepG2 cells proliferation(P