Optimization of purification technology of naringin from leaves of Acanthopanax evodiaefolius by response surface methodology / 中草药

Objective: Response surface methodology was used to optimize the purification process of naringin from Acanthopanax evodiaefolius leaves by polyamide resin. Methods: The optimum technological conditions for the purification of naringin in the leaves of Acanthopanax evodiaefolius were screened by single factor investigation and response surface design with five factors, including the concentration of sample, sample loading, the elution system, the amount of eluent, and the flow rate. Results: The optimum purification conditions of naringin in the leaves of A. evodiaefolius were as follow: the concentration of the sample was 4.0 mg/mL, the sample volume was 3.5 BV, the elution system was 30% methanol, the eluant volume was 3.0 BV, and the elution flow rate was 8.0 BV/h. Under this condition, the purity of naringin was improved from 5.08% to 56.12%, and the yield was 41.69%. And mass fraction reached more than 90% after recrystallization, which met the requirements of pharmaceutical raw materials. Conclusion: Purification of naringin from the leaves of A. evodiaefolius by polyamide resin chromatography has the advantages of good purification effect, simple operation, high efficiency, and good stability, which can be used for industrial production.

Chemical constituents from leaves of Acanthopanax evodiaefolius / 中草药

Objective: To study the chemical constituents from the leaves of Acanthopanax evodiaefolius. Methods: The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified on the basis of their physicochemical properties and spectroscopic data. Results: Thirteen compounds were isolated from the methanol extract of A. evodiaefolius and their structures were identified as heptadecanoic acid (1), heptacosanol (2), trans-cinnamic acid (3), ursolic acid (4), oleanolic acid (5), betulinic acid (6), corosolic acid (7), apigenin (8), naringenin (9), maltol (10), syringic acid (11), stigmasterol (12), and daucosterol (13), respectively. Conclusion: Compounds 1-3 and 7-10 are obtained from the plans in genus Acanthopanax Miq. for the first time and all the compounds are obtained from A. evodiaefolius for the first time.