Correlation between active components alignment and medicinal function of Peony and Licorice decoction / 国际药学研究杂志

Objective: To study the change regulation of nine compounds(oxypaeoniflorin, albiflorin, paeoniflorin, benzoyl-paeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin) in Peony and Licorice decoction, and analyze the correlation between therapeutical effects and active components alignment. Methods: Classical prescription Peony and Licorice decoction (Shanghanlun) were taken as the carrier, five different combinations of Peony and Licorice decoction were used as the control, and the content was determined by HPLC-DAD. Results: The contents of nine compounds of different combinations of Peony and Licorice decoction was(mg/ml): Shanghanlun 1.92, 0.90, 3.51, 0.53, 8.35, 3.16, 0.77, 0.77 and 0.15; Chuanxinshiyongfang 0.92, 0.45, 1.62, 0.37, 0.78, 0.34, 0.06, 0.10 and 0.01, Zhushijiyanfang A 1.59, 0.82, 3.28, 0.49, 3.15, 1.10, 0.27, 0.44 and 0.08, Zhushijiyan-fang B 1.14, 0.52, 2.12, 0.41, 2.92, 0.97, 0.22, 0.36 and 0.06, Weishijiacangfang 1.38, 0.59, 2.70, 0.43, 2.40, 0.35, 0.06, 0.10 and 0.01, Yi-menbafang 1.52, 0.67, 2.91, 0.45, 3.00, 1.07, 0.24, 0.42 and 0.07. Conclusion: 1. The change regulation of the nice compounds was Shang-hanlun>Zhushi-jiyanfang A > Yimenbafang, oxypaeoniflorin, albiflorin, paeoniflorin and benzoylpaeoniflorin in Zhushijiyanfang B Weishijia cangfang. chuanxinshiy-ongfang has the the lowest content of the nine compounds. 2. Total contents and proportion of the nine compounds are different due to different compatibility. The clinical curative effect is also different. Total contents of the nine compounds in Shanghanlun were 20.06 mg/ml which is used to relieve pain in the legs, in Zhushijiyanfang A was 8.72 mg/ml which is used to relieve pain caused by diabetes, in Zhushijiyanfang B was 8.72 mg/ml which is used to treat foot weakness, in Chuanxinshiyong-fang was 4.65 mg/ml which is used to treat wet beriberi, in Weishijiacangfang was 8.02 mg/ml which is used to treat wet beriberi, in Yimenbafang was 10.35 mg/ml which is used to treat peratodynia.

Analysis on changing rules of four active components alignment in Glycyrrhizae Radix et Rhizoma Praeparata cum Melle and its compatibility carrier / 中草药

Objective: To study the changing rules of Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (GRRPM) with the active components alignment (ACA, liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin) of tonifying spleen and replenishing qi. Methods: Taking single herbs, their medicine pairs, or their medicine compounds as test sample, the sample solution was prepared by traditional water decoction and determined by HPLC. Results: The contents of liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin in GRRPM were (12.14±0.31), (3.25±0.19), (38.75±1.77), and (1.40±0.25) mg/mL; The ACA contents in GRRPM-Ginseng Radix et Rhizoma (GRR) were (2.87±0.04), (0.74±0.02), (6.42±0.18), and (0.37±0.03) mg/mL; The ACA contents in GRRPM-Atractylodis Macrocephalae Rhizoma (AMR) were (5.99±0.01), (2.16±0.09), (22.40±1.80), and (0.77±0.02) mg/mL; The ACA contents in Sijunzi Decotion (SD) were (2.99±0.03), (0.81±0.04), (10.05±0.31), and (0.32±0.03) mg/mL. Conclusion: Four main active components in the efficacy of the test components have almost the same change rule: GRRPM≤GRRPM-AMR≤SD≤GRRPM-GRR; The percentage change intervals of the ACA (liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin) are (7.76-9.34):(2.00-2.81):(17.35-31.41):1. The total contents in single herbs are three times as much as in compatibility components, so the Chinese materia medica (CMM) are often used in the type of formula, which indicates that the utilized space of ACA resources is larger, ACA has the obvious effect of correspondence, and the quality standard of CMM should be marked ACA and medicinal components of biological potency as indicators.

Research of Quality Evaluation Method of Liyan Dan Complex Pharmaceutics by Biological Effect Ratio / 现代生物医学进展

Objective: To determine biological effect ratio (BER) on anti-herpes simplex virus-Ⅰ (HSV-Ⅰ)of Liyan Dan (LYD) complex pharmaceutics and its active components alignment (ACA), and to discuss the new identification method of Chinese patent medicine expressed by standard BER. Methods: Protective effect to cells infected by HSV-Ⅰ was evaluated by a 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The ACA was determined by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry. By the protective percent (PP) to cells infected by HSV-Ⅰ of LYD and its ACA the BER was calculated. BERS= PPS / PPACA (protective percent to cells infected by HSV-Ⅰ of sample/protective percent to cells infected by HSV-Ⅰ of ACA; the concentration of sample was 2-x mg/mL). Results: LYD can inhibit cytopathogenic effect (CPE) and its BER was 1.16. Conclusion: BER and ACA of Chinese patent medicine can reflect connotation of Chinese patent medicine quality. This index described intrinsic quality of curative effect on Chinese materia medica.The new concepts of BER and ACA were pulled into the study. This research offered a new train of thought and method to study Chinese patent medicine quality as follows. Firstly, establish standard biological effect of ACA as a standard of effect; secondly,measure the biological effect of sample by the method of measuring effective standard of ACA; thirdly, calculate BER according to the formula (BERS=PPS/PPACA). Standard of BER on quality evaluation is as follows. If 1 ≥ BERS ≥ 0.9, the sample is eligible; if BERS＞1, the sample is of high quality; if BERS＜0.9, the sample is disqualification. This quality evaluation method is much more scientific than the method that evaluate quality only by one or two components contents.

Research of Quality Evaluation Method of Liyan Dan Complex Pharmaceutics by Biological Effect Ratio / 现代生物医学进展

Objective: To determine biological effect ratio (BER) on anti-herpes simplex virus-Ⅰ (HSV-Ⅰ)of Liyan Dan (LYD) complex pharmaceutics and its active components alignment (ACA), and to discuss the new identification method of Chinese patent medicine expressed by standard BER. Methods: Protective effect to cells infected by HSV-Ⅰ was evaluated by a 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The ACA was determined by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry. By the protective percent (PP) to cells infected by HSV-Ⅰ of LYD and its ACA the BER was calculated. BERS= PPS / PPACA (protective percent to cells infected by HSV-Ⅰ of sample/protective percent to cells infected by HSV-Ⅰ of ACA; the concentration of sample was 2-x mg/mL). Results: LYD can inhibit cytopathogenic effect (CPE) and its BER was 1.16. Conclusion: BER and ACA of Chinese patent medicine can reflect connotation of Chinese patent medicine quality. This index described intrinsic quality of curative effect on Chinese materia medica.The new concepts of BER and ACA were pulled into the study. This research offered a new train of thought and method to study Chinese patent medicine quality as follows. Firstly, establish standard biological effect of ACA as a standard of effect; secondly,measure the biological effect of sample by the method of measuring effective standard of ACA; thirdly, calculate BER according to the formula (BERS=PPS/PPACA). Standard of BER on quality evaluation is as follows. If 1 ≥ BERS ≥ 0.9, the sample is eligible; if BERS＞1, the sample is of high quality; if BERS＜0.9, the sample is disqualification. This quality evaluation method is much more scientific than the method that evaluate quality only by one or two components contents.

Research of Quality Evaluation Method of Liyan Dan Complex Pharmaceutics by Biological Effect Ratio / 现代生物医学进展

Objective: To determine biological effect ratio (BER) on anti-herpes simplex virus-Ⅰ (HSV-Ⅰ)of Liyan Dan (LYD) complex pharmaceutics and its active components alignment (ACA), and to discuss the new identification method of Chinese patent medicine expressed by standard BER. Methods: Protective effect to cells infected by HSV-Ⅰ was evaluated by a 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The ACA was determined by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry. By the protective percent (PP) to cells infected by HSV-Ⅰ of LYD and its ACA the BER was calculated. BERS= PPS / PPACA (protective percent to cells infected by HSV-Ⅰ of sample/protective percent to cells infected by HSV-Ⅰ of ACA; the concentration of sample was 2-x mg/mL). Results: LYD can inhibit cytopathogenic effect (CPE) and its BER was 1.16. Conclusion: BER and ACA of Chinese patent medicine can reflect connotation of Chinese patent medicine quality. This index described intrinsic quality of curative effect on Chinese materia medica.The new concepts of BER and ACA were pulled into the study. This research offered a new train of thought and method to study Chinese patent medicine quality as follows. Firstly, establish standard biological effect of ACA as a standard of effect; secondly,measure the biological effect of sample by the method of measuring effective standard of ACA; thirdly, calculate BER according to the formula (BERS=PPS/PPACA). Standard of BER on quality evaluation is as follows. If 1 ≥ BERS ≥ 0.9, the sample is eligible; if BERS＞1, the sample is of high quality; if BERS＜0.9, the sample is disqualification. This quality evaluation method is much more scientific than the method that evaluate quality only by one or two components contents.

Comparison of active components alignment from two kinds of compatibility proportion in Puerariae Decoctions / 中成药

AIM:To analyze the changes in the active components alignment of Puerariae Decoctions between its two different compatibility proportion. METHODS: Reversed phase high-performance liquid chromatography (RP-HPLC) was applied. RESULTS: When the compatibility ratio of Radix Puerariae Lobatea, Radix Scutellariae, Rhizoma Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 5∶3∶3∶2, the contents of the eight drug active components alignment were 2.48% puerarin, 0.101% daidzin, 0.614% baicalin, 0.155% berberine, 0.048% palmatine, 0.094% jateorhizine, 0.154% glycyrrhizic acid, 0.283% liquiritin, and the proportional relationship of compatibility was 51.7∶2.1∶12.8∶3.2∶1∶2∶3.2∶5.9. When the compatibility ratio of Radix puerariae lobatae, Radix scutellariae, Radix Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 8∶3∶3∶2, the proportion of contents of the eight active components alignment were 1.67%, 0.143%, 0.36%, 0.085%, 0.031%, 0.048%, 0.126%, 0.174%. And the proportional relationship of compatibility was 54.4∶4.7∶11.7∶2.6∶1∶1.5∶4.1∶5.7. CONCLUSION: When the compatibility percentage consists of 8∶3∶3∶2, only the puerarin content relatively increases. The active components alignment of the rest have no significant difference. This illustrates the contents of the active components alignment of puerariae decoction is related to the compatibility percentage and herb solubility.