RESUMO
Objective:To study the inhibitory effect and anti-cancer mechanisms of adenovirus-mediated ING4 gene on the MG-63 osteosarcoma xenografts in nude mice.Methods:Ad-ING4 was transfected into QBI-293 cells and harvested.15 nude mice of the subcutaneous tumor models were established with MG-63 osteosarcoma cells and were randomly divided into PBS,Ad-GFP and Ad-ING4 groups.Then PBS(100 μl),Ad-GFP(100 μl,10~9pfu/ml) and Ad-ING4 (100 μl,10~9pfu/ml) for each one were given respectively QOD for 5 times,with intratumor injections.Tumor volume changes were monitored;and the 15 mice were sacrificed 2 weeks after treatment,the tumors were removed,weighed and ratios of tumor-suppression were calculated.The morphological changes of apoptotic tumor cells were observed under microscope.Bcl-2,Bax,Caspase-3,VEGF,CD34 expression was tested by immumohistochemistry.Results:High titer(10~9pfu/ml)adenoviral vector of ING4 gene were obtained.In nude mice bearing MG-63 osteosarcoma xenografts,the growth of MG-63 tumors treated by intratumoral injecting of Ad-ING4 was significantly suppressed,compared with PBS group and Ad-GFP group.The ratios of tumor weight-suppression of Ad-ING4 group was 59.3%(P＜0.05).Immumohistochemistry displayed that the expression of Bax,Caspase-3 was up-regulated and the expression of Bcl-2,VEGF,CD34 was down-regulated by Ad-ING4.Conclusion:Ad-ING4 can inhibit the growth of MG-63 osteosarcoma xenografts in nude mice,which may be via activating the apoptosis pathway and inhibiting tumor angiogenesis.
RESUMO
Objective:To investigate the effect of IGN4 gene trasfection on SGC-7901 cells,and the possible mechanism for its anti-tumor effect .Methods:Ad-ING4 was obtained by gene recombination and packaging technique in vitro.The expression of ING4 mRNA and protein were analyzed by RT-PCR and western-blot respectively.The effect of Ad-ING4 on growth of SGC-7901 cells was detected by MTT.Apoptotic cells were detected by Laser Scan Co-focal Microscope (LSCM).The expression of p21 in SGC-7901 cells was analyzed by western-blot.Results:After infection with Ad-ING4,the expression of ING4 mRNA and protein were showed in SGC-7901 cells detected.The proliferation of SGC-7901 cells was inhibited significantly(P