Effect of prednisone on expression of Ezrin and NEPH1 in rats with Adriamycin-induced nephrosis / 中华实用儿科临床杂志

Objective To explore the mechanism of proteinuria and renal protection of prednisone by observing the expressions of Ezrin and NEPH1 in rats with Adriamycin (ADR)-induced nephrosis.Methods The 24 rats were divided into 3 groups,which were control group,model group and prednisone group.ADR model was induced by a tail intravenous injection of ADR for 2 times.Serum index and 24 h urinary protein were measured in 4 and 8 weeks.The expressions of Ezrin and NEPH1 in glomerulus were evaluated by using two-step immunohistochemistry respectively.Results Compared with control group,heavy proteinuria,hypoalbuminemia,hyperlipemia were observed in 4 weeks in the model group and the prednisone group which indicated that the models were successfully established.Compared with model group,in 8 weeks,24 h urinary protein,total cholesterol and serum creatinine in prednisone group were significantly decreased(all P ＜ 0.0 l),while albumin,total protein and endogenous creatinine clearance rate were significantly increased(all P ＜ 0.05) ;The expressions of Ezrin and NEPH1 in model group were significantly decreased compared with control group(all P ＜ 0.01).The expressions of Ezrin and NEPH1 in prednisone group were significantly increased compared with model group(all P ＜0.01).The expressions of Ezrin and NEPH1 were negatively related to proteinuria(r =-0.838,-0.884,all P ＜ 0.01).Conclusions The declined expressions of Ezrin and NEPH1 in rats with ADR-induced nephrosis may be one of mechanisms of proteinuria.Prednisone can reduce the proteinuria and relieve the renal pathological damage by improving the expression of Ezrin and NEPH1.

Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells / 中医杂志

Objective To study the effect of Haikun Shenxi Capsule(HSC)on endothelin-1(ET-1)and mRNA expression of rats with adriamyein-induced nephrosis and proliferation of glomerular mesangial cells(GMCs)cultured in vitro.Methods The rats were randomized into 6 groups,normal group,sham operation group,model group,benazepril group,HSC low-and high-dose groups.Ten weeks after treatment,ET-1 and mRNA expression were measured with immunohistochemical and hybridization in situ method.Tak- ing the Benazepril group as the control group,the proliferation of GMCs was detected with MTT method.Results In the model group,ET-1 and mRNA expression presented a strong positive,compared with which,that of the HSC low-and high-dose groups was remarkably reduced(P