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Objective To establish the spatial course of distal tubule and afferent arterioles after macula densa, and to locate and detect the proteins in the adjacent parts by using three-dimensional visualization technology of microstructure. Methods C57 BL/6J mice were fixed by perfusion and embedded in epon 812. Tissue blocks were cut perpendicular to the longitudinal axis of the kidney. And a total of 720, 2. 5 μm-thick consecutive sections were obtained from the renal capsule to the outer stripe of the renal outer medulla. After aligning the digital microscopic images through computer registration procedures, the tubules and vessels were traced by 3D reconstruction program edited by C Language. Selecting the tissue sections of the contact site and applying the improved immunoperoxidase staining method to detect H
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Objective To compare the pathological changes of the kidney in two-kidney one-clip hypertension and spontaneous hypertension rats so as to provide some experimental basis for clinical prevention and treatment of primary and secondary hypertension. Methods Conventional histopathology and elastic staining methods were used to observe the morphological changes of kidney tissues and renal arterioles from two-kidney one-clip (2K1C) and spontaneously hypertensive rats (SHR) of different weeks of age. Results The blood pressure of 2K1C rats after 4 weeks of operation reached (187?24)mmHg,which was significantly higher than that of controls (P