The research of liver failure in Banna miniature pigs caused by amanita exitialis / 中华劳动卫生职业病杂志

Objective: To explore the characteristics of Banna miniature pig liver failure induced by amanita exitialis. Methods: From September to October 2020, a reverse high performance liquid chromatography (RP-HPLC) method was used to determine the toxin content of amanita exitialis solution, and 2.0 mg/kg amanita exitialis solution (α-amanitins+β-amanitins) was administered orally to Banna miniature pigs. Toxic symptoms, blood biochemical indexes and histopathological changes of liver, heart and kidney were observed at each time point. Results: All Banna miniature pigs died within 76 h of exposure, and different degrees of digestive tract symptoms such as nausea, vomiting and diarrhea appeared between 6 and 36 h. The biochemical indexes of alanine aminotransferase, aspartate aminotransferase, total bilirubin, lactate dehydrogenase, myoglobin, creatine kinase isoenzyme, blood urea nitrogen and creatinine increased significantly at 52 h after exposure, and the differences were statistically significant compared with 0 h (P<0.05). The bleeding of liver and heart was obvious under macroscopic and microscopic observation, hepatocyte necrosis, renal tubule epithelial cell swelling. Conclusion: Large dose of amanita exitialis can cause acute liver failure of Banna miniature pigs, which is in line with the pathophysiological characteristics of acute liver failure, and lays a foundation for further research on the toxic mechanism and detoxification drugs of amanita exitialis induced liver failure.

Clinical study of severe acute mushroom poisoning with myocardial damage / 重庆医科大学学报

Objective:To explore severe acute mushroom poisoning with myocardial damage and the prognosis of the patient. Method: The clinical data of 18 cases of severe acute mushroom poisning treated from 1998 to 2007 in my hospital were collected and analyed retrospectively,and the biochemical indicators were compared. Results:18 cases of severe acute mushroom poisoning not only presented the gastrointestinal tract,liver,kidney toxicity,but particularly prominent cardiac toxicity,mainly as increase of myocardial enzymes, electrocardiogram changes and malignant arrhythmia. Given conventional detoxication,myocardial protection,anti-arrhythmia,blood purification treatment and so on,the patients showed a significant decline in serum concentrations of CR,BUN,ALT,AST,LDH,CK, CK-MB,and HBDH. The differences between pre-treatment and post-treatment indicators in both groups were significant (P